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以洛巴伊大口蘑为试材,采用袋栽方法,测定了洛巴伊大口蘑在棉籽壳培养基上生长期间栽培基质的降解及其相关胞外酶活性变化,以了解洛巴伊大口蘑对碳源的降解利用特点。结果表明:洛巴伊大口蘑具有完整的胞外纤维素酶系;淀粉酶在菌丝生长阶段活性较高;羧甲纤维素酶、滤纸纤维素酶和半纤维素酶的活性与纤维素和半纤维素的降解速率呈正相关,但β?葡萄糖苷酶活性与纤维素的降解速率没有相关性,漆酶和过氧化物酶活性与木质素的降解速率没有相关性;洛巴伊大口蘑具有很强的木质素降解能力,属于白腐菌;非木质纤维素主要在菌丝生长阶段被利用,木质纤维素是子实体生长发育阶段的主要碳源;在107d的栽培过程中,培养基失重52.40%,其中呼吸消耗43.98%,子实体转化率为8.42%。
Taking Lobaii largemouth mushroom as test material, the bag-planting method was used to determine the degradation of culture substrate and the changes of extracellular enzyme activity during the growth of Lobaiyi macrophylla in cottonseed shell medium, Degradation of carbon source characteristics. The results showed that: Lobaiyi mushroom has a complete extracellular cellulase system; amylase activity in mycelial growth stage higher; carboxymethylcellulase, filter paper cellulase and hemicellulase activity and cellulose and The degradation rate of hemicellulose was positively correlated, but the activity of β-glucosidase had no correlation with the degradation rate of cellulose. The activity of laccase and peroxidase had no correlation with the degradation rate of lignin. Strong lignin degradation, belonging to the white-rot fungi; non-lignocelluloses are mainly used in the mycelial growth stage, lignocellulosic fruiting body growth and development stage of the main carbon source; in the 107d cultivation process, the medium weightlessness 52.40%, of which respiratory consumption was 43.98%, fruiting body conversion rate was 8.42%.