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目的:探讨正电子凋亡显像剂n 18F-2-（5-氟-戊基）-2-甲基丙二酸（n 18F-ML-10）检测顺铂诱导的肺腺癌A549细胞凋亡情况的可行性。n 方法:将肺腺癌A549细胞分为对照组、顺铂不同作用时间组、顺铂不同剂量组，其中对照组细胞不加顺铂；顺铂不同作用时间组加入50 μg/ml顺铂分别作用12、18、24、30、36、42、48 h，对照组细胞培养48 h；顺铂不同剂量组分别采用10、20、30、40、50、60、70、80、90、100 μg/ml顺铂作用30 h。采用流式细胞术检测细胞凋亡情况，计算各组凋亡细胞n 18F-ML-10标准摄取值。n 结果:随着50 μg/ml顺铂作用时间的延长，A549细胞凋亡率逐渐增加（n F=66.87，n P<0.01），n 18F-ML-10标准摄取值也逐渐增加（n F=86.47，n P<0.01）。顺铂作用时间为48 h时细胞凋亡率达到最高[（63.10±14.00）%]，n 18F-ML-10标准摄取值也达到最高（4.97±1.03）（均n P<0.01）。顺铂作用30 h后，随着顺铂剂量的增加，细胞凋亡率、n 18F-ML-10标准摄取值均逐渐增加（均n P<0.01），顺铂100 μg/ml组细胞凋亡率最高[（37.31±2.48）%]，n 18F-ML-10标准摄取值最高（3.08±0.20）。n 结论:采用n 18F-ML-10检测顺铂诱导的A549细胞凋亡具有可行性。n “,”Objective:To investigate the feasibility of positron apoptosis radioactive tracer n 18F-labeled 5-fluoropentyl-2-methyl-malonic acid (n 18F-ML-10) in the detection of cisplatin inducing apoptosis of lung adenocarcinoma A549 cells.n Methods:Lung adenocarcinoma A549 cells were divided into the control group, cisplatin time groups and cisplatin dose groups. Cisplatin was not added to the control group. Cisplatin time groups with added 50 μg/ml cisplatin were used for 12, 18, 24, 30, 36, 42 and 48 h, respectively, and the cells of the control group were cultured for 48 h; cisplatin dose groups were treated with 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 μg/ml cisplatin, respectively for 30 h. The apoptosis was detected by using flow cytometry, and the n 18F-ML-10 uptake rate of apoptotic cells in each group was calculated.n Results:With the prolongation of the action time of 50 μg/ml cisplatin, the apoptosis rate of A549 cells was increased gradually ( n F = 66.87, n P < 0.01), and the standavd uptate value of n 18F-ML-10 was also increased gradually (n F = 86.47, n P < 0.01). When cisplatin was treated for 48 h, the apoptosis rate [(63.10±14.00)%] and n 18F-ML-10 standard uptake value (4.97±1.03) reached the highest (all n P < 0.01). After cisplatin treatment for 30 h, with the increase of cisplatin dose, the apoptosis rate and n 18F-ML-10 standavd uptate value were gradually increased (all n P < 0.01), and the apoptosis rate of cisplatin 100 μg/ml group was the highest [(37.31±2.48)%], and the n 18F-ML-10 standavd uptate value was the highest (3.08±0.20).n Conclusions:18F-ML-10 is feasible in the detection of cisplatin inducing the apoptosis of A549 cells.n