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目的观察容量过负荷致慢性心力衰竭大鼠血浆及心肌组织基质金属蛋白酶-8(MMP-8)及其抑制物-1(TIMP-1)的表达变化,探讨其在慢性心力衰竭发病中的病理生理作用。方法雄性SD大鼠17只,随机分为分流组(n=9)和对照组(n=8)。分流组通过腹主动脉下腔静脉穿刺术建立容量过负荷致慢性充血性心力衰竭动物模型,对照组大鼠除不做穿刺外,余操作过程同分流组。分别测定2组大鼠心功能及血流动力学指标,检测血浆MMP-8及TIMP-1水平,实时荧光定量PCR测定大鼠左心室、右心室MMP-8 mRNA、TIMP-1 mRNA的表达。结果术后8周,分流组大鼠左心室收缩压、左心室舒张压、左心室内压差、左心室内压最大上升速率及最大下降速率较对照组明显降低(Pa<0.05,0.01);左心室舒张末压较对照组明显升高(P<0.05)。分流组大鼠血浆MMP-8、TIMP-1水平均较对照组明显升高(Pa<0.05)。与对照组相比,分流组大鼠左心室心肌组织MMP-8 mRNA及左、右心室心肌组织TIMP-1 mRNA水平均有升高趋势,右心室MMP-8 mRNA水平有下降趋势,但2组比较差异均无统计学意义(Pa>0.05);左心室和右心室心肌组织中MMP-8/TIMP-1明显降低,右心室较左心室下降更明显。结论 MMP-8与TIMP-1通过影响胶原代谢,参与容量过负荷致慢性充血性心力衰竭的病理生理过程。
Objective To observe the changes of the expression of matrix metalloproteinase-8 (MMP-8) and its inhibitor of metalloproteinase-1 (TIMP-1) in plasma and myocardium of rats with chronic heart failure induced by volume overload and to explore its pathological changes in the pathogenesis of chronic heart failure Physiological role. Methods Seventeen male SD rats were randomly divided into shunt group (n = 9) and control group (n = 8). The shunt group established an animal model of chronic congestive heart failure caused by volume overload through the inferior vena cava puncture of the abdominal aorta. In the control group, except the non-puncture, the rest of the procedure was the same as the shunt group. The levels of MMP-8 and TIMP-1 in plasma and the expression of TIMP-1 mRNA in left ventricle and right ventricle were measured by real-time fluorescence quantitative PCR. Results Compared with the control group, the left ventricular systolic pressure, left ventricular diastolic pressure, left ventricular pressure, maximum rate of left ventricular pressure and maximum rate of descent in the shunt group were significantly decreased (P <0.05, 0.01). The left ventricular end diastolic pressure was significantly higher than that of the control group (P <0.05). The levels of plasma MMP-8 and TIMP-1 in the shunt group were significantly higher than those in the control group (Pa <0.05). Compared with the control group, the level of MMP-8 mRNA in left ventricular myocardium and the level of TIMP-1 mRNA in left ventricle and right ventricle of right ventricular in both groups were increased and the level of MMP-8 mRNA in right ventricle was decreased (P> 0.05). The levels of MMP-8 / TIMP-1 in the left ventricle and the right ventricle were significantly lower than those in the left ventricle. Conclusion MMP-8 and TIMP-1 are involved in the pathophysiological process of chronic congestive heart failure by affecting collagen metabolism.