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采用组织化学和免疫组织化学技术 ,研究大鼠幽门括约肌内NOS ,AChE ,VIP和CGRP阳性神经元成分的分布及其在甲醛致胃炎性痛时的变化 ,拟阐明括约肌内肠神经系统 (ENS)中一些神经成分与胃肠运动功能的相互关系。结果发现 :①正常括约肌黏膜下层有散在的含AChE和NOS神经元胞体。括约肌肌层有含NOS和AChE神经丛 ,内含数个神经元胞体 ;也可见个别CGRP和VIP免疫反应性神经元胞体。肌层内四种阳性纤维丰富。在肌纤维增厚的括约肌部位这些神经元成分特别丰富。②甲醛致胃炎性痛鼠 ,括约肌黏膜下神经丛及肌间神经丛NOS ,AChE ,VIP神经元阳性反应产物 (平均光密度 )均比对照组显著降低 (P <0 .0 1) ,但CGRP较对照组升高 (P <0 .0 1)。结果提示 :ENS中上述这些神经元成分可能参与幽门括约肌活动和胃肠运动的调控机制。
To investigate the distribution of NOS, AChE, VIP and CGRP-positive neurons in the pyloric sphincter of rats and their changes in the formation of gastritis induced by Formaldehyde, histochemistry and immunohistochemistry were used to elucidate the relationship between the sphincteric nervous system (ENS) Correlation between some neurological components and gastrointestinal motor function. The results showed that: ① normal sphincter submucosa scattered with AChE and NOS neurons cell body. Sphincter muscle contains NOS and AChE nerve plexus, contains several neuronal soma; also see individual CGRP and VIP immunoreactive neuronal soma. Muscle four positive fibers rich. These neurons are particularly rich in sphincter muscle that thickens in muscle fibers. (2) The positive reaction products (average optical density) of NOS, AChE and VIP neurons in submucous plexus and sphincter of gastric mucosa induced by formaldehyde were significantly lower than those in control group (P <0.01), but CGRP Compared with the control group (P <0.01). The results suggest that the above neuron components in ENS may participate in the regulation of pyloric sphincter activity and gastrointestinal motility.