论文部分内容阅读
目的:建立同时测定丹参素(DSS)、羟基红花黄色素A(HSYA)、芍药苷(PF)、阿魏酸(FA)血浆样品HPLC分析方法,研究血必净注射液中主要有效成分DSS、HSYA、PF、FA在大鼠体内的药代动力学。方法:尾静脉注射给药,不同时间采集血样,HPLC测定各成分血药浓度,用DAS 2.0软件拟合药时曲线,对药代动力学参数进行分析。结果:DSS、HSYA、PF、FA的回收率均大于72%;日内、日间精密度RSD均小于6%;DSS、HSYA、PF、FA的t1/2β分别为(12.206±4.211)min,(54.207±11.516)min,(23.397±8.553)min,(16.482±5.896)min;VF分别为(5.673±1.954)L·kg-1,(0.587±0.375)L·kg-1,(0.866±0.586)L·kg-1,(21.263±2.698)L·kg-1;CL分别为(0.237±0.093)L·min-1,(0.020±0.005)L·min-1,(0.033±0.009)L·min-1,(1.707±0.364)L·min-1。结论:该方法简便、准确,可用于血必净注射液的质量控制,适合多组分药代动力学研究,为血必净的临床用药提供依据。
Objective: To establish a method for the simultaneous determination of plasma samples of Danshensu (DSS), hydroxysafflor yellow A (HSYA), paeoniflorin (PF) and ferulic acid (FA), and to study the main effective component DSS in Xuebijing injection. Pharmacokinetics of HSYA, PF and FA in rats. Methods: Blood samples were collected by tail vein injection and blood samples were collected at different times. The plasma concentrations of each component were determined by HPLC. The pharmacokinetic parameters were analyzed with DAS 2.0 software. Results: The recoveries of DSS, HSYA, PF and FA were all greater than 72%. The intra-day and inter-day precision RSD were all less than 6%. The t1/2β of DSS, HSYA, PF and FA were (12.206±4.211) min, respectively ( 54.207±11.516) min, (23.397±8.553) min, (16.482±5.896) min; VF were (5.673±1.954) L·kg-1, (0.587±0.375) L·kg-1, (0.866± 0.586)L·kg-1, (21.263±2.698)L·kg-1; CL was (0.237±0.093)L·min-1, (0.020±0.005)L·min-1, (0.033±0.009)L, respectively. Min-1, (1.707±0.364) L·min-1. Conclusion: The method is simple and accurate. It can be used for the quality control of Xuebijing injection. It is suitable for the study of multi-component pharmacokinetics and provides the basis for the clinical use of Xuebijing.