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目的克隆大鼠及人肝再生增强因子的ｃＤＮＡ．方法按文献报道大鼠及人肝再生增强因子核苷酸序列设计合成引物．利用ｍＲＮＡ抽提试剂盒分别从乳鼠及人胎肝组织中提取ｍＲＮＡ，再逆转录聚合酶链反应扩增出需要的ｃＤＮＡ片段，经克隆入ｐＧＥＭＴ质粒后以Ｔ７ＤＮＡ聚合酶序列分析试剂盒测定ｃＤＮＡ的序列．结果经ＲＴＰＣＲ反应顺利扩增到４７０ｂｐ的鼠肝再生增加因子ｃＤＮＡ及３８４ｂｐ的人肝再生增强因子．结论所得到的ｃＤＮＡ序列与文献报道一致
Objective To clone cDNA of rat and human augmenter of liver regeneration. Methods The synthetic primers were designed according to the reported nucleotide sequences of rat and human liver regeneration augmentation factor. Using mRNA extraction kit were extracted from rat liver and human fetal liver mRNA, and then reverse transcriptase polymerase chain reaction amplification of the required cDNA fragments, cloned into pGEM T plasmid T7 DNA polymerase sequence analysis kit The sequence of cDNA was determined. Results The RT-PCR reaction was successfully expanded to 470bp rat liver regeneration factor cDNA and 384bp human liver regeneration factor. Conclusion The cDNA sequence obtained is consistent with that reported in the literature