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研究了茜素蓝S与氯霉素的显色反应,建立了测定氯霉素的可见分光光度法。在酸性Britton-Robinson缓冲介质中,茜素蓝S与氯霉素反应生成的络合物导致吸光强度(ΔA)显著增大。最大正吸收波长位于638nm,表观摩尔吸光系数(ε)为1.32×104L·mol-1·cm-1;最大负吸收波长为566nm,表观摩尔吸光系数(ε)为8.00×103L·mol-1·cm-1;氯霉素的浓度在0—2.0×10-5mol.L-1范围内遵从比耳定律。探讨了适宜的反应条件、主要分析化学性质及方法的精密度和可靠性。方法用于市售氯霉素药物中氯霉素的测定,结果满意。
The color reaction between alizarin blue S and chloramphenicol was studied, and a spectrophotometric method was established for the determination of chloramphenicol. In acid Britton-Robinson buffer medium, the complex formed by the reaction of alizarin blue S with chloramphenicol results in a significant increase in absorbance (ΔA). The maximum positive absorption wavelength was at 638nm, the apparent molar absorptivity (ε) was 1.32 × 104L · mol-1 · cm-1, the maximum negative absorption wavelength was 566 nm, the apparent molar absorptivity (ε) was 8.00 × 103L · mol- 1 · cm-1. The concentration of chloramphenicol obeys Beer’s law in the range of 0-2.0 × 10-5mol.L-1. The suitable reaction conditions were discussed, mainly analyzing the chemical properties and the precision and reliability of the method. The method was applied to the determination of chloramphenicol in chloramphenicol drugs with satisfactory results.