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目的探讨一起院内新生儿腹泻暴发的病原及其特征。方法常规方法对可疑腹泻暴发住院新生儿患者粪便、医护人员肛拭子、婴儿奶粉和医院环境标本进行病原分离和鉴定,将分离到的病原菌进行毒力基因检测、药敏试验及PFGE和MLST分子分型分析。结果从12名住院新生儿腹泻患者黄色水样便和1份新生儿ICU病房配奶间的环境标本中分离出13株大肠杆菌O128∶H45和1株大肠杆菌O55,13株大肠杆菌O128∶H45耐热肠毒素基因st均为阳性、具有高度相似性的PFGE带型、MLST的型别均为ST2332型且具有相似的耐药谱。结论首次报道了由肠产毒性大肠杆菌O128∶H45引起的一起院内新生儿腹泻暴发。
Objective To investigate the etiology and characteristics of neonatal diarrhea in hospital. Methods The routine method was used to isolate and identify the pathogenic bacteria from the excrement of hospitalized neonates, anal swabs, infant formula and hospital environmental specimens of suspicious diarrhea outbreaks. The virulence genes, drug sensitivity test and PFGE and MLST molecules Typing Analysis. Results Thirteen strains of Escherichia coli O128: H45 and one strain of Escherichia coli O55 and 13 strains of Escherichia coli O128: H45 were isolated from 12 samples of neonatal diarrhea hospitalized with yellow water and one newborn ICU ward with milk. Heat-stable enterotoxin gene st are positive, with a high degree of similarity PFGE band type, MLST type are ST2332-type and have similar resistance spectrum. Conclusions For the first time an outbreak of nosocomial neonatal diarrhea was reported in the study of enterotoxigenic Escherichia coli O128: H45.