作者在以药用人参中分离精制抗肿瘤物质人参三醇的同时,开发了特异性的、极敏感的酶免疫测定法(ELISA)。为抗体所识别的部分是二乙炔及乙二醇。运用ELISA法,以鉴定人参三醇或其类似物质为目的,对30种生药进行了筛选。 实验结果:将30种生药的甲醇提取物分别用50mM磷酸缓冲液(pH7.4)做10~2,10~3,10~4倍系列稀释,运用ELISA测定的结果发现,几种芹菜科、菊科的生药显示浓度依赖性 The authors developed a specific and extremely sensitive enzyme immunoassay (ELISA) while separating and purifying the anti-tumor substance ginseng triol in medicinal ginseng. The part identified for the antibody is diacetylene and ethylene glycol. Thirty crude drugs were screened for the purpose of identifying Panaxatriol or its analog using ELISA. Experimental results: The methanol extracts of 30 crude drugs were serially diluted 10-2, 10-3, and 10-4 times with 50 mM phosphate buffer (pH 7.4). The results of ELISA assay showed that several celery families, The crude drug of Compositae shows concentration dependence