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目的 :探讨人肝癌肝组织存在卵圆细胞的可能性。方法 :对 2 0例人肝细胞肝癌的手术标本行常规组织学和超微结构观察 ,并用胆管上皮分化标记CK7和肝细胞分化标记白蛋白对以上组织作免疫组化染色 ,同时对其中 5例作免疫电镜标记。结果 :光镜下 ,14/ 2 0例癌肿边缘常可见到增生的小胆管样结构。电镜下 ,14/ 2 0例可找到三型卵圆细胞。其中Ⅰ型细胞体积较小、核大、胞质少 ,此为较为原始的卵圆细胞。Ⅱ型细胞体积稍大 ,胞质稍多 ,此为向胆管上皮分化的小上皮细胞。Ⅲ型细胞体积稍大 ,内含稍多粗面内质网 ,此为向肝细胞分化的卵圆细胞。此三型卵圆细胞均有细胞内张力微丝和细胞间连接结构。免疫电镜显示 ,三型卵圆细胞均表达CK7和白蛋白 ,但Ⅰ型和Ⅱ型表达CK7多些 ,Ⅲ型表达白蛋白多些。结论 :与动物致癌模型肝中存在卵圆细胞一样 ,人肝细胞肝癌肝中也存在同样形态和免疫表型特点的卵圆细胞。结果支持卵圆细胞可能为肝前体细胞的假设。
Objective: To explore the possibility of the presence of oval cells in human liver cancer. METHODS: Conventional histological and ultrastructural observations of 20 surgical specimens of human hepatocellular carcinoma were performed. Immunohistochemical staining of the above tissues was performed using bile duct epithelial differentiation marker CK7 and hepatocyte differentiation marker albumin. For immunoelectron microscopy markers. RESULTS : Under the light microscope, hyperplastic bile duct-like structures were often seen on the edge of 14/20 cancers. Under electron microscope, 14/20 cases can find type III oval cells. The type I cells are smaller in size, larger in nucleus and less in cytoplasm, which is more primitive oval cells. Type II cells are slightly larger and have slightly more cytoplasm. This is a small epithelial cell that differentiates into bile duct epithelium. Type III cells are slightly larger and contain slightly more rough endoplasmic reticulum, an oval cell that differentiates into hepatocytes. The three types of oval cells all have intracellular tension microfilaments and intercellular connecting structures. Immunoelectron microscopy showed that all types of oval cells expressed CK7 and albumin, but type I and type II expressed more CK7, and type III expressed more albumin. Conclusion: Oval cells with the same morphology and immunophenotypic characteristics are present in the liver of human hepatocellular carcinoma as well as the presence of oval cells in the animal carcinogenesis model. The results support the hypothesis that oval cells may be hepatic precursor cells.