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目的：测定硝普钠（ＳＮＰ）及环鸟苷酸（ｃＧＭＰ）对培养的小脑神经原的细胞毒性和亚甲蓝（ＭＢ）对其小鼠毒性的预防作用．方法：用Ｄｅｓｉ法测定ＳＮＰ和ｃＧＭＰ的小鼠小脑神经细胞毒性．测定ｉｃｖＳＮＰ的小鼠惊厥发生率和２４ｈ死亡率及ＭＢ对其毒性的预防作用．结果：给予ＳＮＰ１ｍｍｏｌ·Ｌ－１１０ｍｉｎ细胞存活率由对照组９２％降至３５％．ｃＧＭＰ０１ｍｍｏｌ·Ｌ－１作用１ｈ，细胞存活率由９４％降至４０％．ＳＮＰ２０ｎｍｏｌｉｃｖ使１／１０小鼠在２４ｈ内死亡，３０ｎｍｏｌ使１１／１３小鼠死亡．ＭＢ１００ｎｍｏｌｉｃｖ可预防１１／１３的ＳＮＰ中毒（３０ｎｍｏｌ，ｉｃｖ）小鼠发生惊厥及死亡，可完全消除ＳＮＰ２０ｎｍｏｌ的中毒效应．结论：ＳＮＰ和ｃＧＭＰ对小鼠小脑神经细胞有细胞毒性．亚甲蓝明显对抗ｉｃｖＳＮＰ小鼠的惊厥和死亡
Objective: To determine the cytotoxicity of sodium nitroprusside (SNP) and cyclic guanosine acid (cGMP) on cultured cerebellar neurons and the preventive effect of methylene blue (MB) on their toxicity in mice. Methods: The neuronal cytotoxicity of SNP and cGMP in cerebellum of mice was determined by Desi method. The incidence of seizures and 24h mortality in icvSNP mice and the preventive effect of MB on their toxicity were determined. Results: The survival rate of cells treated with SNP 1 mmol·L-110 min decreased from 92% in control group to 35% in control group. The effect of cGMP01mmol·L-1 for 1h decreased the cell viability from 94% to 40%. One in 10 mice died within 24 h with SNP 20 nmolicv and 11/13 in mice with 30 nmol. MB100nmolicv can prevent SNP poisoning (30nmol, icv) in 11/13 mice from seizures and death and completely eliminate SNP 20nmol poisoning effect. Conclusion: SNP and cGMP are cytotoxic to mouse cerebellar neurons. Methylene blue significantly counteracted seizures and death in icvSNP mice