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目的探讨槲皮素对不同时间和不同剂量染矽尘大鼠血清中硅(Si)、铜(Cu)、锌(Zn)元素水平的影响。方法采用正交设计,将108只无特定病原体级健康成年雄性SD大鼠随机分成对照组,矽肺模型组,以及按正交设计表L16(43)安排不同染毒剂量(分别为12.5、25.0和50.0 g/L)、不同染毒时间(分别为7、14、21和28 d)、槲皮素干预(分别为12.5、25.0和50.0 mg/kg)各水平组成的16个实验条件组。实验结束后,各组大鼠经心脏采血,采用电感耦合等离子体-质谱法测定血清中Si、Cu和Zn元素水平,并进行肺组织病理学检查。结果予槲皮素干预的大鼠肺泡炎及肺间质纤维化均较矽肺模型组有不同程度的减轻。矽肺模型组大鼠血清中Si、Cu元素水平和Cu/Zn比值均高于对照组(P<0.05)。不同剂量单纯染尘的正交8、11和14组大鼠血清Si元素水平均高于对照组(P<0.05)。不同剂量槲皮素干预的正交5、6、7、9、10和13组大鼠血清Si元素水平均低于矽肺模型组(P<0.05)。予槲皮素干预的正交5、13组大鼠血清Cu元素水平和Cu/Zn比值均低于矽肺模型组(P<0.05)。各组大鼠血清Zn元素水平分别两两比较,差异均无统计学意义(P>0.05)。结论槲皮素可以延缓实验性矽肺早期病变的形成和发展,Cu和Cu/Zn比值的变化可初步作为矽肺疗效的评价指标。
Objective To investigate the effect of quercetin on the levels of silicon (Si), copper (Cu) and zinc (Zn) in sera of rats exposed to silica dust at different times and different doses. Methods A total of 108 healthy male SD rats without specific pathogen were randomly divided into control group, silicosis model group and orthogonal design table L16 (43), with different exposure doses (12.5, 25.0 and 50.0 g / L), 16 different experimental conditions at different time points (7, 14, 21 and 28 d) and quercetin intervention (12.5, 25.0 and 50.0 mg / kg, respectively) At the end of the experiment, blood was collected from the heart of rats in each group, and the levels of Si, Cu and Zn in serum were determined by inductively coupled plasma-mass spectrometry, and lung histopathological examination was performed. Results Quercetin intervention of rat alveolitis and interstitial fibrosis than silicosis model group to varying degrees, reduce. The levels of Si, Cu and Cu / Zn in serum of silicotic model group were higher than that of the control group (P <0.05). The serum levels of Si in 8, 11 and 14 groups with different doses of purely dust were higher than those in the control group (P <0.05). The levels of Si in serum of 5, 6, 7, 9, 10 and 13 groups were significantly lower than those of silicosis model (P <0.05). The levels of Cu and Cu / Zn in the serum of the Orthogonal Groups 5 and 13 that received quercetin intervention were lower than those of the silicosis model group (P <0.05). The levels of Zn in serum of each group were respectively compared with each other, the differences were not statistically significant (P> 0.05). Conclusions Quercetin can delay the formation and development of experimental silicosis, and the change of Cu and Cu / Zn ratio may be used as an index to evaluate the efficacy of silicosis.