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目的:在动物体内检验多西环素纳米脂质体缓释凝胶的生物相容性及其对牙周损伤动物模型的修复效果。方法:毒性实验-用20%样品(多西环素纳米脂质体缓释凝胶)水溶液给予SD大鼠灌胃,观察长期应用多西环素纳米脂质体凝胶剂有无全身毒性反应及其程度。造模动物实验-将牙周损伤动物模型造模成功的SD大鼠分为5组(空白组、派丽奥组、纳米组、基质组、造模组),按分组进行相应治疗,通过实时定量PCR检验各组大鼠龈沟液中MMP-8及其抑制剂TIMP-1的表达,采用SAS13.3软件包对数据进行t检验。结果:自制的多西环素纳米脂质体缓释凝胶具有良好的生物相容性;经多西环素纳米脂质体缓释凝胶治疗1个月后的牙周病变大鼠龈沟液中MMP-8含量明显降低,与其他对照组相比有显著性差异(P<0.05)。结论:牙周袋内应用多西环素纳米脂质体缓释凝胶可降低龈沟液中MMP-8的含量,减轻牙周炎症,提示该药物制剂对改善大鼠牙周炎症有一定疗效。
OBJECTIVE: To examine the biocompatibility of doxycycline nano-liposome sustained-release gel in vivo and its effect on the animal model of periodontal injury. Methods: Toxicity test - SD rats were orally administered with 20% aqueous solution of doxycycline nano-liposomes (PDGF) and observed for long-term application of doxycycline nano-liposome gel with or without systemic toxicity And its extent. Animal model experiment - Sprague-Dawley rats were divided into 5 groups (blank group, Pelio group, nano group, stroma group and model group), which were treated according to grouping. Real-time Quantitative PCR was used to detect the expression of MMP-8 and its inhibitor TIMP-1 in gingival crevicular fluid in each group. The SAS13.3 software package was used for t-test. Results: The homemade doxycycline nano-liposome sustained-release gel has good biocompatibility; the gingival sulcus of rats with periodontal lesions treated with doxycycline nano-liposome sustained-release gel for 1 month The content of MMP-8 in the fluid was significantly lower than that in other control groups (P <0.05). Conclusion: The application of doxycycline nano-liposome sustained-release gel in periodontal pouch can reduce the content of MMP-8 in gingival crevicular fluid and reduce periodontal inflammation, suggesting that the drug has a certain curative effect on improving periodontal inflammation in rats .