不同金属离子对产氢发酵细菌Biohydrogenbacterium R3 sp.nov.发酵产氢能力的影响

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研究浓度为0.5mg/L的ZnSO4、CuSO4、CoCl2、CaCl2、Fe0、FeSO4以及不同浓度梯度下CoCl2对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响.研究发现,当反应器内分别添加0.50mg/L的ZnSO4、CuSO4、CoCl2、CaCl2、Fe0、FeSO4时,Biohydrogenbacterium R3 sp.nov.的生长和产氢效能与对照组相比均有一定程度的提高.综合比较不同金属离子对Biohydrogenbacterium R3 sp.nov.的产氢及生长效能的促进作用,当反应器内添加0.50mg/L的FeSO4时,Biohydrogenbacterium R3 sp.nov.的氢气产量达到最大值228mL/L,此时的细胞浓度为0.43g/L,而添加了CoCl2的反应器内Biohydrogenbacterium R3 sp.nov.的细胞浓度达到最大值0.87g/L,此时的氢气产量为191.5mL/L.CoCl2的浓度在0.05~0.50mg/L范围内时,当CoCl2浓度为0.05mg/L时,氢气产量达到最大值205mL/L,而当CoCl2浓度在0.30mg/L时,细胞浓度达到最大值1.03g/L,当CoCl2浓度在1.00~2.00mg/L范围内时,Biohydrogenbacterium R3 sp.nov.的氢气产量与细胞浓度均开始下降,产生了高浓度抑制现象.
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