论文部分内容阅读
目的调查不同区域空气中苯并[a]芘(B[a]P)暴露情况,探讨接触B[a]P对3~5岁学龄前儿童尿液神经递质含量的影响。方法利用高效液相色谱法测定重庆市主城区某焦化厂附近不同区域(分别距焦化厂1 000、2 000、3 000、4 000m)、不同季度空气中B[a]P暴露浓度;收集出生于该地区且出生以来居住地固定的3~5岁学龄前儿童尿液样本,用ELISA方法测定儿童尿液中谷氨酸(Glu)、γ-氨基丁酸(GABA)、多巴胺(DA)和5-羟色胺(5-HT)的含量,分析B[a]P暴露变化与儿童尿液神经递质含量改变的关系。结果空气B[a]P浓度随着与污染源距离变大而降低,同一季度4个不同区域整体比较差异有统计学意义(P<0.05),同一区域不同季度整体比较差异也有统计学意义(P<0.05)。儿童尿液神经递质(Glu、GABA、DA、5-HT)在同一暴露范围内随着年龄的增大而含量升高,在同一年龄段4种神经递质的含量随着B[a]P暴露浓度的升高而降低。结论 B[a]P暴露可降低3~5岁儿童尿液神经递质Glu、GABA、DA、5-HT含量,其因果关联还需要进一步调查研究。
Objective To investigate the exposure of benzo [a] pyrene (B [a] P) in the air of different regions and to explore the effect of exposure to B [a] P on the neurotransmitter contents in urine of 3 ~ 5 years old preschool children. Methods The concentrations of B [a] P in air of different quarters (1 000, 2 000, 3 000 and 4 000 m respectively from the coking plant) and the air of different quarters were measured by high performance liquid chromatography (HPLC) Urine samples of preschool children aged 3-5 years old living permanently in the area and living in the area were assayed for urinary glutamate (Glu), gamma-aminobutyric acid (GABA), dopamine (DA) and 5 - serotonin (5-HT) content, analysis of B [a] P exposure changes and the relationship between urinary neurotransmitter content in children. Results The concentration of B [a] P in the air decreased with the increase of the distance from the source of pollution. There were significant differences among the four different regions in the same quarter (P <0.05), and there was significant difference in the same region in different seasons (P <0.05). Urine neurotransmitters (Glu, GABA, DA, 5-HT) in children with the same exposure range increased with age, the content of the four neurotransmitters at the same age as B [a] P exposure concentration decreased. Conclusion Exposure to B [a] P can reduce urinary neurotransmitter levels of Glu, GABA, DA and 5-HT in children aged 3 to 5 years. The causal relationship between B [a] P exposure and further investigation is needed.