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硫酸庆大霉素注射液、硫酸阿米卡星注射液和硫酸西索米星注射液是临床上常用的氨基糖苷类抗生素。《中国药典》2000年版二部收载了这3种注射抗生素,其鉴别方法均采用薄层色谱法,其展开条件和展开后显色方法各不相同。笔者在检验中发现,硫酸阿米卡星制剂按《中国药典》收载的方法薄层色谱,层析结果并不理想,供试品与标准品所显的主斑点Rf值明显偏小,拖尾严重,展开时间过长(约2～3h),点样量过大,造成主斑点过大,不够集中。经反复试验筛选,试用展开剂由氯仿-甲醇-浓氨-水(1:4:2:1)改为氯仿-甲醇-浓氨-丙酮(10:
Gentamicin sulfate injection, amikacin sulfate injection and sisomicin sulfate injection is clinically used aminoglycoside antibiotics. The “Chinese Pharmacopoeia” 2000 edition of the two contained three injection antibiotics, the identification methods are used thin-layer chromatography, the expansion conditions and color method developed after each different. The author found in the test, amikacin sulfate preparations according to “Chinese Pharmacopoeia” method of loading thin-layer chromatography, the chromatographic results are not ideal for the test samples and standards showed the main spot Rf value was significantly smaller, drag Tail serious start time is too long (about 2 ~ 3h), sample size is too large, resulting in the main spot is too large, not focused. After repeated testing and screening, the test developing solvent was changed from chloroform-methanol-concentrated ammonia-water (1: 4: 2: 1) to chloroform-