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本文以肝细胞线粒体呼吸速率(S)、呼吸控制率(RCR)、氧化磷酸化效率(ADP/O)和肝脏ATP含量为指标测定了大鼠急性梗阻性胆管炎(AOC)时线粒体呼吸功能的变化,并观察了异搏停和地塞米松对线粒体损害的防治效果。结果发现,AOC术后6h时线粒体Ⅳ态呼吸速率(S_4)即有明显增强,RCR、ADP/O和肝脏ATP含量明显降低,而异搏停和地塞米松对AOC的治疗组在术后48h内各指标均无明显变化。结果提示,AOC早期即能够引起严重的肝细胞线粒体呼吸功能损害和能量代谢障碍,异搏停和地塞米松能够有效地防治AOC所致的肝细胞线粒体呼吸功能和能量代谢障碍。
In this paper, mitochondrial respiration rate (A), respiratory rate (S), respiratory control rate (RCR), oxidative phosphorylation efficiency (ADP / O) and hepatic ATP content were measured in mitochondria of rats with acute obstructive cholangitis Changes, and observed the verapamil and dexamethasone mitochondrial damage control effect. The results showed that the mitochondrial IV state respiratory rate (S_4) was significantly increased 6 h after AOC, and the contents of RCR, ADP / O and hepatic ATP were significantly decreased. However, the AOC treated with verapamil and dexamethasone for 48 h No significant changes in the indicators. The results suggest that early stage of AOC can cause severe damage of mitochondrial respiratory function and energy metabolism in hepatocytes. Verapamil and dexamethasone can effectively prevent and treat mitochondrial respiratory function and energy metabolism disorder caused by AOC.