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目的研究紫杉醇脂质体体外对人直肠癌Colo320细胞的影响。方法以0.01、0.1、1、10、100μmol/L等浓度梯度的紫杉醇脂质体作用于人直肠癌Colo320细胞,并设立空白对照组,通过MTT法和TUNEL法等技术检测和分析紫杉醇脂质体药对Colo320细胞增殖和凋亡的影响。结果上述不同浓度的紫杉醇脂质体作用于直肠癌Colo320细胞24～96h,随着作用时间的延长和药物浓度的升高,细胞存活率逐渐降低,作用于Colo320细胞24h,随着药物浓度的升高,细胞凋亡指数逐渐升高。结论紫杉醇脂质体体外可以抑制人直肠癌Colo320细胞的增殖,促进细胞的凋亡。
Objective To study the effect of paclitaxel liposomes on human colorectal cancer cell line Colo320 in vitro. Methods paclitaxel liposomes with equal concentration gradient of 0.01, 0.1, 1, 10 and 100μmol / L were used to act on human colorectal cancer Colo320 cells and a blank control group was set up. The paclitaxel liposomes were detected and analyzed by MTT assay and TUNEL assay Effect of Drugs on Proliferation and Apoptosis of Colo320 Cells. Results The different concentrations of paclitaxel liposomes act on colorectal cancer cells Colo320 24 ~ 96h, with the extension of time and drug concentration, cell viability decreased gradually, the role of Colo320 cells 24h, with the drug concentration High, the apoptotic index gradually increased. Conclusion Paclitaxel liposomes can inhibit the proliferation of human colorectal cancer Colo320 cells in vitro and promote cell apoptosis.