本文介绍了一种新的锝标记蛋白质方法,并与应用Iodogen的放射性碘标记、锡还原法的~(99m)Tc标记和通过DTPA螯合的~(111)In标记等法作了比较。标记方法:将~(99m)Tc-高锝酸盐(200μl,在生理盐水中)、二甲基甲酰胺(11μl)和5N盐酸(3μl)混合,在140℃加热4h。用氯仿(200μl)提取干燥后的残留物,并移到另一试管内蒸发至干,得到中间物。在该中间物中加入200μl将要标记的蛋白质溶液(1～10mg/ml,用生理盐水配成),在40℃ In this paper, a new technetium-labeled protein was introduced and compared with Iodogen’s radioactive iodine labeling, ~ (99m) Tc labeling by tin reduction and ~ (111) In labeling by DTPA chelation. Labeling Method: ~ (99m) Tc-pertechnetate (200μl in physiological saline), dimethylformamide (11μl) and 5N hydrochloric acid (3μl) were mixed and heated at 140 ° C for 4h. The dried residue was extracted with chloroform (200 μl) and transferred to another tube and evaporated to dryness to give the intermediate. To this intermediate, 200 μl of protein solution to be labeled (1 to 10 mg / ml, formulated with physiological saline) was added and the mixture was incubated at 40 ° C