用国产显色三肽CBZ-gly-pro-arg-pNA为底物测定血浆纤溶酶原(plg)活性,选择链激酶(SK)为plg激活物,向待测标本中加入足量的SK与plg形成具有纤溶酶活性的复合物,对实验系统中的显色底物产生酰胺分解作用,裂解出显色集团对硝基苯胺。血浆中plg活性与显色反应呈正相关。作者对实验的最佳条件和影响因素作了讨论。用本法测定60例健康者plg活性,提出正常参考值范围为100.00±8.69%(■±S)。 Plasminogen activator (pgg) activity was determined by using the domestic chromogenic tripeptide CBZ-gly-pro-arg-pNA as the substrate, and selecting for the streptokinase (SK) With the formation of fibrinolytic complexes plg, the experimental system of chromogenic substrate amide decomposition, cleavage of the color group p-nitroaniline. PLg plasma activity and color reaction was positively correlated. The author discusses the optimal conditions and influencing factors of experiment. Using this method to determine the activity of plg in 60 healthy people, the normal reference range was proposed to be 100.00 ± 8.69% (± s).