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目的探讨阿司匹林预防去势大鼠(卵巢切除)骨质疏松的作用及机制。方法取48只3月龄SD雌性大鼠并随机分为6组:去势组(OVX组)、对照组(Sham组)及4个不同剂量阿司匹林干预组,每组8只。OVX组及4个阿司匹林组均行卵巢切除术。去势后1周,阿司匹林干预组分别按2.25、4.46、8.92及26.75mg/kg(A1、A2、A3及A4组)灌胃,1次/d。OVX组及Sham组予等量0.9%氯化钠注射液灌胃,1次/d。灌胃3个月后观察血清骨源性碱性磷酸酶及骨钙素水平;以双能X线吸收骨密度测量仪(DXA)测量L5椎体骨密度;对股骨骨小梁微观结构、胃黏膜应激情况行组织病理学观察;用Micro-CT分析腰椎椎体骨小梁微观三维形态结构。结果与OVX组比较,A3、A4组骨源性碱性磷酸酶显著降低(P<0.05)。A2、A3、A4组骨钙素与Sham组相比显著升高(P<0.05)。DXA分析结果显示,阿司匹林各剂量组BMD值与OVX组差异有统计学意义(P<0.01)。病理组织形态学显示阿司匹林各剂量组骨小梁形态结构较OVX组好;A3、A4组胃黏膜可见黏膜红斑及黏膜下出血,A4组最明显。Micro-CT分析表明,与OVX组比较,阿司匹林各剂量组BV/TV、Tb.Th、Tb.N、BMD均显著提高(P<0.01),BS/BV、Tb.Sp显著降低(P<0.01),阿司匹林各剂量组BV/TV、BS/BV、Tb.Th、Tb.N、Tb.Sp、BMD与Sham组相比差异有统计学意义(P<0.01)。结论阿司匹林可以改善去势大鼠骨小梁结构,增加骨质密度,可用于预防绝经后骨质疏松的发生,其机制可能是通过抑制骨吸收及刺激骨形成发挥作用,为小剂量阿司匹林可能用于防治骨质疏松提供了实验依据。
Objective To investigate the effect and mechanism of aspirin on prevention of osteoporosis in ovariectomized rats. Methods Forty-eight SD female rats of 3 months old were randomly divided into 6 groups: OVX group, Sham group and 4 different doses of aspirin intervention group, 8 rats in each group. OVX group and 4 aspirin group underwent ovariectomy. One week after castration, the aspirin intervention group was administered intragastrically at doses of 2.25, 4.46, 8.92 and 26.75 mg / kg (groups A1, A2, A3 and A4) once daily. OVX group and Sham group were given the same amount of 0.9% sodium chloride injection, 1 / d. Serum bone alkaline phosphatase and osteocalcin levels were observed 3 months after gavage. BMD of L5 vertebrae was measured by dual energy X-ray absorptiometry (DXA). The microstructure of trabecular bone and stomach Mucosal stress histopathological observation; micro-CT analysis of lumbar vertebral trabecular micro three-dimensional morphology. Results Compared with OVX group, the bone alkaline phosphatase of A3 and A4 groups was significantly decreased (P <0.05). The levels of osteocalcin in A2, A3 and A4 groups were significantly higher than those in Sham group (P <0.05). DXA analysis showed that the aspirin BMD value of each dose group and OVX group difference was statistically significant (P <0.01). Histopathology showed that trabecular bone morphological structure was better in all doses of aspirin than that in OVX group. In group A3 and A4, erythema and submucosal hemorrhage were observed in gastric mucosa, and the most obvious was in group A4. Micro-CT analysis showed that BV / TV, Tb.Th, Tb.N and BMD in aspirin groups were significantly increased (P <0.01) and Tb / BV and Tb.Sp were significantly decreased in OVX group BV, TV, BS / BV, Tb.Th, Tb.N, Tb.Sp, BMD in aspirin groups were significantly different from Sham group (P <0.01). Conclusion Aspirin can improve trabecular structure and increase bone density in ovariectomized rats, which may be used to prevent osteoporosis in postmenopausal rats. The mechanism may be that bone resorption and bone formation may be inhibited by aspirin, which may be useful for low-dose aspirin In the prevention and treatment of osteoporosis provides an experimental basis.