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目的 直接克隆 p5 3下游基因 ,并对其功能进行初步研究。方法 采用哺乳动物细胞诱导表达系统 -Tet-onTM基因表达系统 ,建立 p5 3基因诱导表达可调控的细胞系 ;通过mRNA差异显示技术直接克隆p5 3下游基因 ,并利用原位杂交技术检测其在小鼠胚胎发育过程中的表达。结果 克隆到一个新的p5 3下游基因侯选基因 ,并检测到它在小鼠胚胎发育过程中有特异性表达。结论 直接克隆 p5 3下游基因的成功 ,为进一步研究 p5 3基因的功能奠定了基础。
Objective To clone the downstream gene of p5 3 and study its function. Methods The mammalian cell-derived expression system-Tet-onTM gene expression system was established to establish a cell line with p5 3 gene expression induction. The downstream gene of p5 3 was cloned by mRNA differential display technology and was detected by in situ hybridization Expression in mouse embryonic development. As a result, a new candidate gene downstream of p5 3 was cloned and its expression was detected during mouse embryonic development. Conclusion The direct cloning of the downstream gene p5 3 laid the foundation for further study on the function of p5 3 gene.