【摘 要】
：
目的探讨HIV-1感染者中枢神经系统(CNS)的病毒学特征。方法通过克隆测序分析病毒亚型,观察CNS中HIV-1变异性和离散率;依据V3环11/25法则并结合10例有偿献血HIV-1感染者尸检脑
【机 构】
：
首都医科大学附属北京佑安医院北京市肝病研究所,
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目的探讨HIV-1感染者中枢神经系统(CNS)的病毒学特征。方法通过克隆测序分析病毒亚型,观察CNS中HIV-1变异性和离散率;依据V3环11/25法则并结合10例有偿献血HIV-1感染者尸检脑组织标本免疫荧光试验分析中枢神经HIV-1复合受体的使用。结果有偿献血人群感染的HIV-1显示B和B′亚型特征,脑脊液(CSF)和外周血序列有嵌合现象;CSF C2-V5区序列变异性明显低于外周血,两者的离散率差异无统计学意义;依据11/25法则分析V3环序列可见中枢神经HIV-1大多数使用CCR5复合受体。结论 CNS HIV-1病毒变异性明显小于外周血;CNS HIV-1具有CCR5嗜性。
Objective To investigate the virological characteristics of central nervous system (CNS) in HIV-1 infected patients. Methods The virus subtypes were analyzed by cloning and sequencing, and the variability and dispersion rate of HIV-1 in the CNS were observed. According to the V3 loop 11/25 rule and the immunohistochemical analysis of autopsy brain tissue of 10 HIV-1 infected donors, -1 complex receptor. Results The HIV-1 infected with blood donors showed the characteristics of B and B subtypes and the mosaicism of cerebrospinal fluid (CSF) and peripheral blood. The sequence variability of CSF C2-V5 was significantly lower than that of peripheral blood The difference was not statistically significant. According to the 11/25 rule analysis of the V3 loop sequence, most of the central nervous system HIV-1 cells used the CCR5 complex receptor. Conclusion The variability of CNS HIV-1 virus is significantly less than that of peripheral blood. CNS HIV-1 has CCR5 tropism.
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