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目的研究黄芪提取物(extract of astragalus,EA)对大鼠局灶性脑缺血(MCAO)再灌注的血脑屏障损伤的影响。方法采用线栓法制备MCAO再灌注模型,观察EA对缺血再灌注大鼠的神经功能障碍、脑梗死体积和脑含水量、外周血循环内皮细胞(CEC)含量、缺血脑组织中细胞间粘附分子(ICAM-1)的表达、缺血侧脑组织皮质血脑屏障超微结构的病理学变化的影响。结果 EA80 mg.kg·1,EA(40,80 mg.kg·1)、EA(20,40,80 mg.kg·1)可分别改善缺血再灌注2,8,24 h大鼠的神经功能障碍;能明显降低外周血中的CEC含量,明显减轻缺血脑组织的脑水肿和脑梗死体积,对脑缺血再灌注后血脑屏障超微结构的病理改变有一定的改善作用;能减少ICAM-1免疫反应阳性血管数。结论 EA对局灶性脑缺血再灌注损伤大鼠有一定的保护作用,作用机制可能与其保护血管内皮细胞,减轻脑缺血再灌注后的血脑屏障损伤有关。
Objective To investigate the effect of extract of astragalus (EA) on the blood-brain barrier injury induced by focal cerebral ischemia (MCAO) in rats. Methods The reperfusion model of MCAO was established by the method of thread embolism. The effects of EA on neurological dysfunction, cerebral infarction volume and brain water content, circulating endothelial cells (CEC), ischemic brain tissue intercellular adhesion (ICAM-1) expression and the pathological changes of the blood-brain barrier ultrastructure in ischemic cortex. Results EA80 mg · kg · 1, EA (40,80 mg · kg · 1) and EA (20,40,80 mg · kg · 1) could improve the neurological function of rats at 2, 8, 24 h after ischemia / reperfusion Dysfunction; can significantly reduce the CEC content in peripheral blood, significantly reduce cerebral edema and cerebral infarction volume of ischemic brain tissue after cerebral ischemia-reperfusion, the pathological changes of the blood-brain barrier ultrastructure can improve; Reduce the number of ICAM-1 immunoreactive blood vessels. Conclusion EA can protect rats from focal cerebral ischemia-reperfusion injury and its mechanism may be related to the protection of vascular endothelial cells and the impairment of blood-brain barrier after cerebral ischemia-reperfusion.