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为了研究纳米三氧化二砷磁性脂质体(NMLA)的热化疗作用对卵巢癌HO-8910细胞nm23H1和c-myc mRNA表达有无影响。以培养液做对照,分别将空白脂质体、三氧化二砷(As2O3)溶液、纳米As2O3脂质体、纳米磁性脂质体(NML)、NMLA作用于人浆液性卵巢癌HO-8910细胞,并用高频交变磁场(AMF)对经过NML和NMLA处理的HO-8910细胞作进一步热疗处理,RT-PCR方法检测各组细胞nm23H1和c-myc的mRNA表达情况。实验发现,As2O3使nm23H1mRNA的表达上调,热疗对其无影响;单纯As2O3及热疗均使c-mycmRNA的表达下调,纳米As2O3磁性脂质体的热化疗对其下调作用更为显著。上述结果表明,NMLA可在高频AMF作用下对卵巢癌细胞进行热化疗,机制与nm23H1和c-myc的表达相关。
In order to investigate whether Nano-arsenic trioxide magnetic liposomes (NMLA) have the effect of thermochemotherapy on the expression of nm23H1 and c-myc mRNA in ovarian cancer HO-8910 cells. The culture medium was used as a control, and blank liposomes, arsenic trioxide (As2O3) solution, nano-As2O3 liposomes, nano-magnetic liposomes (NML) and NMLA were respectively treated on human serous ovarian cancer HO-8910 cells, Alternating magnetic field (AMF) was used to further treat HO-8910 cells treated with NML and NMLA, and RT-PCR was used to detect the mRNA expression of nm23H1 and c-myc in each group. The results showed that As2O3 could up-regulate the expression of nm23H1mRNA and had no effect by hyperthermia. The expression of c-myc mRNA was down-regulated by both As2O3 and hyperthermia, and the thermotherapy with nano-As2O3 magnetic liposomes was more effective. The above results show that NMLA can be used in the thermo-chemotherapy of ovarian cancer cells under the action of high-frequency AMF. The mechanism is related to the expression of nm23H1 and c-myc.