【摘 要】
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Epididymitis is a commonly diagnosed disease associated with male infertility.However,little is known about the molecules that are involved in its development.This study was to identify critical genes associated with lipopolysaccharide-induced epididymiti
【机 构】
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Department of Urology, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiao Tong Universit
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Epididymitis is a commonly diagnosed disease associated with male infertility.However,little is known about the molecules that are involved in its development.This study was to identify critical genes associated with lipopolysaccharide-induced epididymitis and analyze the molecular mechanism of epididymitis through RNA sequencing.Experimental epididymitis models were generated by administering male Sprague-Dawley rats\' lipopolysaccharide.A total of 1378 differentially expressed genes,including 531 upregulated and 847 downregulated genes,were identified in the epididymitis model rats compared with those in sham-operated rats by RNA sequencing.Functional enrichment analyses suggested that the upregulated genes were markedly enriched in inflammationrelated biological processes,as well as in the tumor necrosis factor (TNF) signaling pathway,cytokine-cytokine receptor interactions,complement and coagulation cascades,and in the chemokine signaling pathway.Four downregulated genes (collagen type ⅩⅩⅧ alpha 1 chain [Col28α1],cyclin-dependent kinase-like 1 [Cdkl1],phosphoserine phosphatase [Psph],and fatty acid desaturase 2 [Fads2]) and ten upregulated genes (CCAAT/enhancer-binding protein beta [Cebpβ],C-X-C motif chemokine receptor 2 [Cxcr2],interleukin 11 [II11],C-C motif chemokine ligand 20 [Ccl20],nuclear factor-kappa-B inhibitor alpha [Nfκbiα],claudin 4 [Cldn4],matrix metallopeptidase 9 [Mmp9],heat shock 70 kDa protein 8 [Hspa8],intercellular cell adhesion molecule-1 [Icam1],and Jun) were successfully confirmed by real-time polymerase chain reaction.Western blot demonstrated that CDKL1 was decreased,while MMP9 and NFKBIA were increased in the experimental model group compared with those in the sham-operated group.Our study sheds new light on the understanding of the early response of the epididymis during bacterial epididymitis.
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