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目的 :观察三氧化二砷 (As2 O3)对人膀胱癌细胞的生长抑制作用 ,并探讨其机制。方法 :采用 MTT法检测不同浓度的 As2 O3对人膀胱癌细胞株 BIU- 87的生长抑制率 ,原位末端转移酶标记技术检测细胞凋亡 ,SABC免疫组织化分析 BIU- 87细胞中 bcl- 2的表达。结果 :As2 O3可有效地抑制 BIU- 87细胞生长 ,具有时间、浓度依赖性特点 ;经药物作用后 ,膀胱癌凋亡细胞明显增多 ,凋亡率随作用时间的延长而增高 ,BIU- 87细胞中的bcl- 2表达显著降低。结论 :As2 O3可显著抑制膀胱癌细胞生长 ;通过下调 bcl- 2基因表达诱导细胞凋亡是其作用机制之一。
Objective: To observe the effect of arsenic trioxide (As 2 O 3) on the growth of human bladder cancer cells and to explore its mechanism. Methods: The growth inhibition rates of human bladder cancer cell line BIU-87 with different concentrations of As2 O3 were detected by MTT assay. Apoptosis was detected by in situ terminal transaminase-labeled technique. The expression of bcl-2 in BIU-87 cells was detected by SABC immunohistochemistry expression. Results: As2 O3 could effectively inhibit the growth of BIU-87 cells in a time and concentration-dependent manner. After drug treatment, the apoptosis of bladder cancer cells was significantly increased and the apoptosis rate increased with the prolongation of time. BIU-87 cells Bcl-2 expression was significantly reduced. CONCLUSION: As2 O3 can significantly inhibit the growth of bladder cancer cells. Inducing apoptosis by down-regulating bcl-2 gene expression is one of its mechanisms.