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目的实验动物的健康状况对科学研究的发展有重要的影响,为了保证实验用鼠的健康质量,自从2009年起本实验室对北京市多家实验动物单位的实验用鼠进行了病理学抽检,从病理学的角度来调查北京市部分实验鼠的主要脏器的情况。方法我们主要对实验鼠的心、肝、脾、肺、肾、大肠和小肠进行取材,通过甲醛固定,石蜡切片HE染色、冰冻切片油红“O”染色、PAS染色等方法对实验用鼠进行病理学分析。结果根据病理学的调查结果,部分实验动物在以肝脏和肾脏为代表的器官中出现了不同程度的病变。结论结合对实验鼠的微生物学检测,分析认为,北京市的啮齿类实验动物中几乎无细菌、病毒和寄生虫等病原微生物的阳性感染个体,对人、动物及周围环境无明显危害;但是少部分实验鼠在临床上出现一定程度的病理学异常。
Objectives The health status of experimental animals has an important influence on the development of scientific research. In order to ensure the health quality of experimental mice, the laboratory conducted pathological sampling of experimental rats from a number of laboratory animal units in Beijing since 2009, From the pathological point of view to investigate some of the major organs of rats in Beijing’s situation. Methods We mainly harvested the heart, liver, spleen, lung, kidney, large intestine and small intestine of rats by HE staining, paraffin section HE staining, frozen section oil red staining, PAS staining and other methods Rats were pathologically analyzed. Results According to the pathological findings, some experimental animals showed varying degrees of pathological changes in organs represented by liver and kidney. Conclusions Based on the microbiological examination of the experimental mice, it is concluded that there are almost no positive individuals infected with pathogenic microorganisms such as bacteria, viruses and parasites in rodent laboratory animals in Beijing, and no obvious harm to humans, animals and the surrounding environment; however, Some rats in the clinic there is a certain degree of pathological abnormalities.