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目的探讨交通相关细颗粒物对CEM-6T细胞凋亡的影响,为研究交通相关细颗粒物的免疫毒性机制提供实验依据。方法使用不同剂量(0、20、80、320μg/ml)交通相关细颗粒物染毒CEM-6T细胞24和48 h,用RT-PCR测定CEM-6T细胞中Caspase-3的mRNA表达,以Western Blot测定Caspase-9、Cyt-c、Fas-L、Caspase-8和TNF-α蛋白表达。结果 320μg/ml交通相关细颗粒物染毒细胞24 h和48 h后,Caspase-3基因表达高于对照组,差异有统计学意义(P<0.05)。80、320μg/ml细颗粒物染毒细胞24和48 h后,细胞内Cyt-C、Caspase-9、Fas-L、Caspase-8、TNF-α蛋白表达升高,与对照组比较,差异有统计学意义(P<0.05)。结论交通相关细颗粒物可通过线粒体通道和死亡受体通道诱导CEM-6T细胞凋亡。
Objective To investigate the effects of traffic-related fine particles on the apoptosis of CEM-6T cells and provide experimental evidence for studying the mechanism of immunotoxicity of traffic-related fine particles. Methods CEM-6T cells were exposed to different concentrations (0, 20, 80 and 320μg / ml) of traffic-related fine particles for 24 and 48 hours respectively. Caspase-3 mRNA expression in CEM-6T cells was detected by RT- The expressions of Caspase-9, Cyt-c, Fas-L, Caspase-8 and TNF-α were measured. Results The expression of Caspase-3 gene was significantly higher in the cells exposed to 320 μg / ml traffic-related fine particles for 24 h and 48 h compared with the control group (P <0.05). The cytotoxicity of Cyt-C, Caspase-9, Fas-L, Caspase-8 and TNF-α in cells exposed to 80 and 320μg / ml fine particles for 24 and 48 hours were significantly higher than those in control group Significance (P <0.05). Conclusion Traffic-related fine particles can induce apoptosis of CEM-6T cells through mitochondrial and death receptor channels.