论文部分内容阅读
目的 探讨重组人促红细胞生成素 (rhEPO )对新生鼠缺氧缺血性脑损伤 (HIBD)的保护作用及对海马CA1区Caspase 3激活的影响。 方法 将 7日龄SD大鼠分为缺氧缺血模型组 (n= 11)、rhEPO治疗组 (n =11)和假手术对照组 (n =10 ) ,模型组和治疗组建立新生鼠缺氧缺血性脑损伤模型。观察模型组和治疗组缺氧后 0、6、12、2 4h不同时间点自发左旋、夹尾左旋、夹尾尖叫现象。用免疫组织化学方法检测活化的Caspase 3的表达 ,观察Caspase 3阳性细胞在脑组织内分布情况 ,计算各组海马CA1区单位面积内阳性细胞数 (个 / 1mm)。 结果 模型组 2只、治疗组 1只在缺氧过程中因持续抽搐死亡。缺氧后 0h两组动物均出现自发左旋 (治疗组 10 / 10 ,模型组 9/ 9) ,2 4h治疗组自发左旋发生率明显减少 (治疗组 1/ 10 ,模型组 6/ 9,P =0 .0 198)。免疫组化显示Caspase 3阳性细胞在对照组脑组织内散在分布 ,模型组和治疗组在海马、大脑皮层较为密集。模型组CA1区阳性细胞数显著高于对照组 (41.3 8± 2 .0 9vs 10 .52± 2 .70 ,P <0 .0 1)。治疗组阳性细胞数显著低于模型组(41.3 8± 2 .0 9vs 2 2 .63± 3 .17,P <0 .0 1)。 结论 Caspase 3在新生鼠HIBD中活性显著增高 ,应用rhEPO能有效抑制Caspase 3激活 ,提示rhEPO通过抑制Casp
Objective To investigate the protective effect of recombinant human erythropoietin (rhEPO) on hypoxic-ischemic brain damage (HIBD) and its effect on activation of Caspase-3 in the hippocampal CA1 region of neonatal rats. Methods 7-day-old SD rats were divided into three groups: hypoxic-ischemic model group (n = 11), rhEPO-treated group (n = 11) and sham operation control group (n = 10) Oxygen-ischemic brain injury model. The model group and the treatment group were spontaneous left-handed, left-handed tail and screaming at different times of 0, 6, 12 and 24 hours after hypoxia. The expression of activated Caspase 3 was detected by immunohistochemical method. The distribution of Caspase 3 positive cells in the brain tissue was observed. The number of positive cells per unit area (1 mm) was calculated. Results Two of the model group and one of the treatment group died of twitch during hypoxia. After 0 h of hypoxia, spontaneous L-levorotatoryity occurred in both groups (10/10 in the treated group and 9/9 in the untreated group), and spontaneous left-handedness was significantly reduced in the 24 h group (1/10 in the treated group and 6/9 in the untreated group, P = 0 .0 198). Immunohistochemistry showed Caspase 3 positive cells scattered in the control group brain tissue, the model group and treatment group in the hippocampus, cerebral cortex is more dense. The number of CA1 positive cells in the model group was significantly higher than that in the control group (41.3 8 ± 2.09 vs 10.52 ± 2.70, P <0.01). The number of positive cells in the treatment group was significantly lower than that in the model group (41.3 8 ± 2.09 vs 22.63 ± 3.17, P <0.01). Conclusion The activity of Caspase 3 in HIBD of neonatal rats is significantly increased. Application of rhEPO can effectively inhibit the activation of Caspase 3, suggesting that rhEPO can inhibit the expression of Caspase