论文部分内容阅读
用使君子酸（ＱＡ）损毁ＳＤ 大鼠左侧Ｍ ｅｙｎｅｒｔ基底大细胞核，制成老年性痴呆症（ＡＤ）模型。将同种鼠胚基底前脑制成的细胞悬液不加神经营养因子和分别加入脑源性神经营养因子（ＢＤＮＦ）、神经生长因子（ＮＧＦ）及ＢＤＮＦ＋ ＮＧＦ者植入ＡＤ 模型鼠额、顶叶皮质，隔日通过脑室灌注人工脑脊液和相应神经营养因子共７ 次。移植后４ 个月，取脑切片作Ｎｉｓｓｌ染色、ＮＡＤＰＨｄ和ＮＡＤＰＨｄ＋ ＡＣｈＥ 组化染色，计数移植区中ＮＯＳ阳性神经元数及其纤维在１６９００ μｍ ２ 网格中的交点数，并用ＭＩＡＳ３００ 计算机图像分析系统对移植区中ＮＯＳ阳性神经元的细胞面积进行处理。结果显示：给予神经营养因子的动物，移植区中的ＮＯＳ阳性神经元数、ＮＯＳ阳性纤维交点数和ＮＯＳ阳性神经元的细胞面积等形态学指标均较不给予神经营养因子的对照组为佳，而在应用神经营养因子的各组中又以ＢＤＮＦ＋ ＮＧＦ组为优，提示ＢＤＮＦ、ＮＧＦ能促进移植区中ＮＯＳ阳性神经元的发育生长，ＢＤＮＦ与ＮＧＦ联合使用可发挥协同作用。本文对ＢＤＮＦ和ＮＧＦ促进移植区中ＮＯＳ阳性神经元发育生长的机制进行了探讨。
Alzheimer’s disease (AD) model was made by damaging the large Meynert basal cell nucleus in the left side of Sprague Dawley rats with QA. The same kind of mouse embryo basal forebrain cell suspension without neurotrophic factor and were added brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and BDNF + NGF were implanted in AD model rat amount, top Leaf cortex, the next day through the ventricle perfusion of artificial cerebrospinal fluid and the corresponding neurotrophic factor a total of 7 times. Four months after transplantation, brain sections were taken for Nissl staining, NADPH-d and NADPH-d + AChE staining. The number of NOS positive neurons in the graft and the number of intersections of the fibers in the 16900 μm 2 mesh were counted, 300 computer image analysis system of transplanted area NOS positive neurons in the cell area for processing. The results showed that morphological indexes such as NOS positive neurons, NOS positive fibers intersections and NOS positive neurons in the transplantation area were better than those without the neurotrophic factor in the animals given neurotrophic factor. However, BDNF + NGF group was the best among the groups with neurotrophic factor, suggesting that BDNF and NGF could promote the development of NOS positive neurons in transplantation area. BDNF and NGF could play synergistic effect. This article explores the mechanisms by which BDNF and NGF promote the development of NOS-positive neurons in transplantation areas.