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目的研究壳聚糖包裹PEB1和IL-21核酸纳米粒子疫苗诱导小鼠免疫应答水平。方法复合物凝聚法制备壳聚糖纳米粒子,肌肉注射Balb/c小鼠,检测免疫期和感染期小鼠血清特异性Ig G水平、脾细胞悬液细胞因子水平、脾细胞增殖指数及疫苗保护率。结果扫描电镜观察纳米颗粒平均粒径在(300±23)nm左右,包封率为(91.23±3.24)%;实验组小鼠,特别是IL-21基因佐剂组,在血清特异性Ig G、脾细胞培养上清中IFN-γ和IL-4、脾细胞增殖水平和疫苗的临床保护效应上都显著高于对照组(P<0.05);但壳聚糖纳米颗粒组效果不明显(P<0.05)。结论 IL-21基因佐剂可以显著提高并维持小鼠稳定持久的免疫应答水平。但壳聚糖对免疫应答仅起到一定的促进作用。本研究的顺利开展,为空肠弯曲菌疫苗的临床应用提供了一定的理论支持。
Objective To study the immunological response of mice immunized with chitosan-encapsulated PEB1 and IL-21 nucleic acid nanoparticle vaccine. Methods Chitosan nanoparticles were prepared by coacervation, and Balb / c mice were intramuscularly injected. Serum specific Ig G level, cytokines level of splenocyte suspension, splenocyte proliferation index and vaccine protection during immunization and infection were detected rate. Results The average particle size of the nanoparticles was (300 ± 23) nm and the encapsulation efficiency was (91.23 ± 3.24)%. The mice in the experimental group, especially the IL-21 gene adjuvant, (P <0.05). However, the effect of IFN-γ and IL-4 in spleen cell culture supernatant and the spleen cell proliferation level and vaccine protective effect were significantly higher than those in control group <0.05). Conclusion IL-21 gene adjuvant can significantly improve and maintain a stable and lasting immune response in mice. However, chitosan can only play a role in promoting the immune response. The successful development of this study provides some theoretical support for the clinical application of Campylobacter jejuni vaccine.