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目的:对铁棒锤和工布乌头进行对比,以证明两者是否存在差异,是否需要区别用药。方法:进行两种药材的显微特征、薄层色谱的比较,采用HPLC法测定铁棒锤及工布乌头中乌头碱的含量。结果:两种药材在显微特征上存在一定的差异。以正己烷:乙酸乙酯:二乙胺(10∶6∶0.8)为展开剂,以乌头碱为对照品,结果 TLC图表明两者成分有明显区别。含量测定结果表明,药材铁棒锤的乌头碱含量为0.71 mg·g-1,而工布乌头中乌头碱的含量仅为0.03 mg·g-1。结论:经过显微、TCL、HPLC的对比研究表明,两种药材有较大差异,在用法上应注意区分,不能将工布乌头视作铁棒锤进行用药。
OBJECTIVE: To compare the iron hammer and cloth Aconitum to prove whether there is a difference between the two, whether the need for distinction between medication. Methods: The microscopic characteristics of two kinds of medicinal materials were compared. The content of aconitine in the aconite was detected by HPLC. Results: There are some differences in the microscopic characteristics of the two medicinal materials. Using n-hexane: ethyl acetate: diethylamine (10:6:0.8) as developing agent and aconitine as reference substance, the result of TLC showed that there was a significant difference between the two components. The results showed that the content of aconitine was 0.71 mg · g-1, while that of aconite was only 0.03 mg · g-1. CONCLUSIONS: The comparative study of microscopic, TCL and HPLC shows that there are great differences between the two medicinal materials. In the usage, attention should be paid to distinguish them.