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[目的]为壳聚糖及壳聚糖铜在农业中的应用提供理论依据。[方法]采用生长速率法测定了壳聚糖及壳聚糖铜对6种植物病原菌的抑菌效果,并建立了毒力回归方程,求出其有效抑制中浓度EC50。[结果]壳聚糖铜对棉花褐斑病菌、黄瓜灰霉病菌和棉花红腐病菌的抑制效果优于壳聚糖,壳聚糖对黄瓜镰刀病菌、凤尾兰炭疽病菌及辣椒根腐病菌的抑制效果优于壳聚糖铜。[结论]壳聚糖及壳聚糖铜均具有较好的抑菌活性,但根据植物病原菌对Cu(Ⅱ)的敏感性不同,应选择合适配比的壳聚糖铜。
[Objective] The research aimed to provide theoretical basis for the application of chitosan and chitosan copper in agriculture. [Method] The antibacterial effect of chitosan and chitosan copper on six kinds of plant pathogens was determined by the growth rate method. The toxicity regression equation was established and the effective concentration of EC50 was also determined. [Result] Chitosan copper was superior to chitosan for inhibiting Cucumber sp., Botrytis cinerea and Rhizoctonia solani. The effect of chitosan on cucumber Fusarium oxysporum, Inhibitory effect is better than chitosan copper. [Conclusion] The chitosan and chitosan copper all had good antibacterial activities. However, according to the different sensitivity of plant pathogens to Cu (Ⅱ), chitosan copper should be selected at a suitable ratio.