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目的:探究东北龙胆对D-氨基半乳糖联合脂多糖诱导的小鼠暴发性肝衰竭的保护作用。方法:小鼠腹腔注射D-氨基半乳糖(700mg/kg)联合脂多糖(10μg/kg)建立小鼠暴发性肝衰竭模型,在模型建立1、12h前给予东北龙胆(50、100、200mg/kg)或水飞蓟素(100mg/kg)。结果:D-氨基半乳糖联合脂多糖导致肝细胞大面积坏死,并能显著升高血清天冬氨酸转氨酶(ALT)、丙氨酸转氨酶(AST)及肿瘤坏死因子α(TNF-α)(P<0.01),减少小鼠肝脏还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSHPx)活性,增加肝脏脂质过氧化物丙二醛(MDA)的水平(P<0.01)。给予东北龙胆可显著改善肝细胞坏死,并减少血清ALT、AST和TNF-α的含量(P<0.01),增加小鼠肝脏GSH、SOD及GSH-Px活性水平(P<0.01)、减少肝脏中MDA含量(P<0.01),缓解由D-氨基半乳糖联合脂多糖引起的肝损伤与氧化应激。东北龙胆还能显著抑制肝脏DNA片段化和半胱氨酸蛋白酶-3(Caspase-3)裂解,并能抑制肝脏中磷酸化c-Jun氨基末端激酶(p-JNK)和磷酸化细胞外信号调节激酶(p-ERK)的表达。结论:对D-氨基半乳糖联合脂多糖所致小鼠暴发性肝衰竭,东北龙胆可通过抑制肝细胞凋亡及阻止肝脏抗氧化状态达到抗肝损伤作用。
Objective: To explore the protective effect of Gentiana scabra against D-galactosamine-induced lipopolysaccharide-induced fulminant hepatic failure in mice. Methods: The mouse model of fulminant hepatic failure was established by intraperitoneal injection of D-galactosamine (700mg / kg) and lipopolysaccharide (10μg / kg). The model mice were given gentian / kg) or silymarin (100 mg / kg). Results: D-galactosamine combined with lipopolysaccharide led to extensive necrosis of hepatocytes and significantly increased serum aspartate aminotransferase (ALT), alanine aminotransferase (AST) and tumor necrosis factor α (TNF-α) P <0.01), reduce the activity of hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSHPx), and increase the activity of hepatic lipid peroxide The level of aldehyde (MDA) (P <0.01). The administration of Gentiana scabra could significantly improve the necrosis of liver cells and decrease the content of serum ALT, AST and TNF-α (P <0.01), and increase the activities of GSH, SOD and GSH-Px in the liver of mice (P <0.01) In MDA content (P <0.01), alleviate the liver injury and oxidative stress caused by D-galactosamine combined with lipopolysaccharide. The gentian also significantly inhibited hepatic DNA fragmentation and caspase-3 cleavage and inhibited the phosphorylation of c-Jun N-terminal kinase (p-JNK) and phosphorylated extracellular signal Regulates kinase (p-ERK) expression. CONCLUSION: Through the inhibition of hepatic cell apoptosis and the prevention of hepatic anti-oxidative status, gentian Gentiana can prevent liver injury in mice with fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide.