论文部分内容阅读
目的:观察外源性低浓度多胺对大鼠缺氧-复氧心肌细胞钙超载的影响。方法:酶解分离大鼠心室 肌细胞,用正常氧合Tyrode液灌流8 min,换为缺氧液灌流32 min,再转换为正常氧合Tyrode液灌流8 min,复制心肌 缺氧-复氧模型。分别在缺氧前给予精胺,缺氧-复氧后给予精胺、精脒、腐胺。应用激光扫描共聚焦显微镜 (LSCM)连续观察细胞内钙荧光强度的动态变化。结果:精胺(1 mol/L)对正常静息状态下大鼠心室肌[Ca2+]i无影 响。缺氧前给予精胺,可取消复氧引起的心肌[Ca2+]i增高;缺氧-复氧后给予精胺、精脒、腐胺对缺氧-复氧引起 [Ca2+]i升高也有不同程度的降低作用,其中以精胺的作用最强。复氧后给予精胺降低缺氧-复氧[Ca2+]i升高的 作用小于缺氧前给予。结论:缺氧前给予精胺可拮抗缺氧-复氧心肌细胞钙超载发生;复氧后给予精胺可使缺氧- 复氧心肌细胞钙超载减轻,但其作用力度不如缺氧前给药。多胺拮抗缺氧-复氧心肌细胞钙超载的作用以精胺> 精脒>腐胺的顺序递减。
Objective: To observe the effect of exogenous low concentration polyamines on calcium overload of hypoxia-reoxygenation cardiomyocytes in rats. Methods: Rat ventricular myocytes were isolated by enzymolysis and perfused for 8 min with normal oxygenated Tyrode solution. Perfused with hypoxic solution for 32 min and then transfused into normal oxygenated Tyrode solution for 8 min, the myocardial hypoxia - reoxygenation model was replicated . Spermine were given before hypoxia, hypoxia - reoxygenation given spermine, spermine, putrescine. The dynamic changes of intracellular calcium fluorescence intensity were observed by laser scanning confocal microscopy (LSCM). RESULTS: Spermine (1 mol / L) had no effect on [Ca2 +] i in normal ventricular myocardium. Spermine administered before hypoxia could eliminate the increase of [Ca2 +] i in myocardium caused by reoxygenation. Spermine, spermidine and putrescine hypoxia-reoxygenation induced increase of [Ca2 +] i after hypoxia-reoxygenation The role of spermine. Spermine administration after reoxygenation reduced the effect of hypoxia-reoxygenation [Ca2 +] i less than before hypoxia. CONCLUSIONS: Spermine administered before hypoxia can antagonize calcium overload in hypoxia-reoxygenation cardiomyocytes. Spermine administration after reoxygenation can reduce calcium overload of hypoxia-reoxygenation cardiomyocytes, but its effect is not as good as hypoxia administration . Polyamine antagonizes calcium overload in hypoxia-reoxygenation cardiomyocytes in the order of spermine> spermine> putrescine.