Differential Proteomic Analysis of Wheat Roots under Different Levels of Potassium

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[Objective] This study aimed to investigate the regulatory role of potassium levels in plant gene expression. [Method] By using wheat (Triticum aestivum L. Beinong 9549) as experimental material, the protein expression in wheat roots under different levels of potassium was determined by two-dimensional electrophoresis (2-DE). [Result] Compared with the plants supplied with normal level of potassium, 152 protein spots in wheat roots under potassium deficiency condition showed significant differences (P<0.05), including 76 protein spots significantly up-regulated by more than twice and 76 protein spots significantly down-regulated by more than twice. After potassium deficiency treatment, the wheat plants were supplied with normal level of potassium for two weeks, which showed significant differences in 73 protein spots compared with the plants continuously supplied with normal level of potassium (P<0.05), including 36 protein spots significantly up-regulated by more than twice and 37 protein spots significantly down-regulated by more than twice. [Conclusion] This study indicates that potassium deficiency stress would lead to induction of a large number of specific proteins in wheat roots. [Objective] This study aimed to investigate the regulatory role of potassium levels in plant gene expression. [Method] By using wheat (Triticum aestivum L. Beinong 9549) as experimental material, the protein expression in wheat roots under different levels of potassium was determined by two-dimensional electrophoresis (2-DE). [Result] Compared with the plants supplied with normal level of potassium, 152 protein spots in wheat roots under potassium deficiency condition showed significant differences (P <0.05) -regulated by more than twice and 76 protein spots significantly down-regulated by more than twice. After potassium deficiency treatment, the wheat plants were supplied with normal level of potassium for two weeks, which showed significant differences in 73 protein spots compared with the plants continuously supplied with normal level of potassium (P <0.05), including 36 protein spots significantly up-regulated by more than twice and 37 pro [Conclusion] This study indicates that potassium deficiency stress would lead to induction of a large number of specific proteins in wheat roots.
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