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目的:建立同时测定暑热宁口服液中麝香草酚、香荆芥酚、百秋李醇、广藿香酮、厚朴酚、和厚朴酚6个有效成分含量的气相色谱方法。方法:采用气相色谱法,色谱柱为HP-5毛细管色谱柱(30m×0.32mm,0.25μm),进样口温度220℃,检测器温度220℃,程序升温,起始温度110℃,保持10min,以25℃程序升温至230℃,保持9.2min,分流比10∶1,进样量1μL。结果:麝香草酚、香荆芥酚、百秋李醇、广藿香酮、厚朴酚、和厚朴酚进样浓度分别在0.226-2.260mg/m L(r=0.99961),0.1055-1.0550mg/m L(r=0.99954),0.212-2.120mg/m L(r=0.99963),0.114-1.140mg/m L(r=0.99955),0.0828-0.8280mg/m L(r=0.99968),0.072-0.720mg/m L(r=0.99975)成良好线性关系,平均加样回收率(n=9)为100.9%-104.5%(RSD2.4%),99.2%-103.4%(RSD1.9%),97.2%-100.8%(RSD1.9%),96.2%-99.4%(RSD1.7%),96.2%-98.7%(RSD2.1%),95.2%-98.2%(RSD1.8%)。结论:该分析方法简单,可控性强,结果准确,可同时分析暑热宁口服液中的多种主要成分。
Objective: To establish a gas chromatographic method for the simultaneous determination of 6 active ingredients of thymidine, celery phenol, periol, patchouli ketone, magnolol and honokiol in Shumingining Oral Liquid. Methods: The column was HP-5 capillary column (30m × 0.32mm, 0.25μm) by gas chromatography. The inlet temperature was 220 ℃ and the detector temperature was 220 ℃. The program temperature was raised and the initial temperature was 110 ℃ for 10min , Programmed temperature was raised to 230 ℃ with 25 ℃, kept 9.2min, split ratio 10: 1, injection volume 1μL. Results: The concentrations of thymol, carvacrol, periol, patchoulen, honokiol and honokiol were 0.226-2.260mg / m L (r = 0.99961), 0.1055-1.0550 (r = 0.99955), 0.0828-0.8280 mg / m L (r = 0.99968), 0.072 mg / m L (r = 0.99954), 0.212-2.120 mg / m L -0.720mg / m L (r = 0.99975). The average recovery was 100.9% -104.5% (RSD 2.4%), 99.2% -103.4% (RSD 1.9%) , 97.2% -100.8% (RSD 1.9%), 96.2% -99.4% (RSD 1.7%), 96.2% -98.7% (RSD 2.1%), 95.2% -98.2% (RSD 1.8%). Conclusion: The analysis method is simple, controllable and accurate, and can simultaneously analyze a variety of main components of Shurening oral liquid.