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目的研究TSPAN-1和CORTACTIN基因在结直肠癌组织中的表达及其与临床病理因素的关系。方法采用免疫组化Elivision二步法,检测TSPAN-1和CORTACTIN基因蛋白在80例结直肠癌组织,13例结肠腺瘤组织及27例癌旁正常结肠黏膜组织中的表达。结果 TSPAN-1蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为7.4%、23.0%、90.0%,组间差异具有统计学意义(P<0.01);CORTACTIN蛋白在正常黏膜、结肠腺瘤、结肠癌组织中阳性表达率分别为52.0%、77.0%、97.5%,组间差异均具有统计学意义(P<0.01);TSPAN-1和CORTACTIN的表达与结肠癌的淋巴结转移、TNM分期、5年生存率、组织学分级及浸润深度有关(P<0.01);TSPAN-1和CORTACTIN在结肠癌组织中的表达强度呈正相关(r=0.397,P=0.0013),二者表达的一致率高达77.8%(62/80)。结论 TSPAN-1和CORTACTIN基因的高表达与结肠癌的侵袭和发展有关,二者在结肠癌的细胞增殖、浸润和转移中有协同作用。联合检测二者的表达对于进一步理解结肠癌的生物学行为和判断预后有一定价值。
Objective To investigate the expression of TSPAN-1 and CORTACTIN in colorectal cancer tissues and their relationship with clinicopathological factors. Methods The immunohistochemical Elivision two-step method was used to detect the expression of TSPAN-1 and CORTACTIN in 80 colorectal cancer tissues, 13 colon adenoma tissues and 27 adjacent normal mucosa tissues. Results The positive expression rates of TSPAN-1 protein in normal mucosa, colon adenoma and colon cancer were 7.4%, 23.0% and 90.0%, respectively (P <0.01). CORTACTIN protein in normal mucosa, colon The positive rates of TSPAN-1 and CORTACTIN in adenomas and colon cancer tissues were 52.0%, 77.0% and 97.5%, respectively. There were significant differences between the two groups (P <0.01) (P <0.01). The expression of TSPAN-1 and CORTACTIN in colon cancer tissues was positively correlated (r = 0.397, P = 0.0013), which was consistent with that of TSPAN-1 and CORTACTIN Rate up to 77.8% (62/80). Conclusion The high expression of TSPAN-1 and CORTACTIN genes is related to the invasion and development of colon cancer. Both of them have a synergistic effect on cell proliferation, invasion and metastasis of colon cancer. Joint detection of the expression of both for further understanding of the biological behavior of colon cancer and determine the prognosis of a certain value.