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利用纯化的空肠弯曲菌表面蛋白PEB1,制备其多克隆抗体。用PEB1蛋白作为抗原免疫兔,分离血清得到兔抗PEB1多克隆抗体,用ELISA法检测抗体效价,并用Western-Blotting验证。PEB1蛋白免疫兔后,得到了兔抗PEB1多克隆抗体,间接ELASA法检测表明兔抗PEB 1血清效价达到1∶10 000,Western印记检测表明,该抗体能与PEB1蛋白发生特异性结合,其特异性与敏感性均较好。获得了高效价的PEB1多克隆抗体。
Polyclonal antibodies were prepared using purified Campylobacter jejuni surface protein PEB1. Rabbit was immunized with PEB1 protein as antigen and polyclonal antibody of rabbit anti-PEB1 was obtained. The antibody titer was detected by ELISA and verified by Western-Blotting. Rabbit anti-PEB1 polyclonal antibody was obtained after immunizing rabbit with PEB1 protein. Indirect ELASA assay showed that the titer of rabbit anti-PEB 1 reached 1:10 000, Western blot analysis showed that the antibody could specifically bind to PEB1 protein. Specificity and sensitivity are better. High-titer PEB1 polyclonal antibody was obtained.