试验采用旋转回归设计方法〔１〕，从１９８６～１９９４年在代表松嫩平原黑土区的东北农业大学试验地内进行了以农肥、化肥、秸秆、绿肥等培肥物质为试验因素的长期定位试验。试验分两个阶段进行，前一阶段为培肥阶段，到１９９２年结束。后一阶段为耗竭阶段，主要观测各培肥处理对土壤养分的持续效应及对玉米产量的影响。建立了耗竭阶段农肥、化肥、秸秆对土壤有机质、全氮、全磷及玉米产量的数学模型〔２〕。试验表明，随着耗竭试验的继续，在产量上和连作不施肥区相比，农肥、化肥、秸秆都有较明显的后效，从两个培肥因素的交互作用上看，农肥与化肥、化肥与秸秆之间有明显的正交互作用，而秸秆和化肥之间也表现了较明显的互补性。 The experiment uses the rotation regression design method [1], from 1986 to 1994 in the northeast agricultural university on behalf of the Songnen Plain black soil area test sites with fertilizer, fertilizer, straw, green manure and other fertilizers as test factors for long-term positioning test. The experiment was conducted in two stages, the previous stage being the stage of fertilization and ending in 1992. The latter phase is the depletion phase, the main observation of the fertilization treatment of soil nutrients continued effect and the impact on corn yield. The mathematical models of soil organic matter, total nitrogen, total phosphorus and corn yield in the depletion phase of agricultural fertilizer, fertilizer and straw were established [2]. Experiments show that with the continuation of the depletion test, compared with continuous cropping and no fertilizing area, there are obvious post-emergence effects of fertilizer, fertilizer and straw. According to the interaction between two fertilizing factors, Fertilizer and straw have a significant positive interaction between straw and fertilizer also showed a more significant complementarity.