用过氧化氢气态等离子体灭活法灭活人类免疫缺陷性病毒Ⅰ型、甲型肝炎病毒、呼吸道合胞病毒、牛痘病毒、单纯疱疹病毒Ⅰ型、脊髓灰质炎病毒Ⅱ型

来源 :国外医学.输血及血液学分册 | 被引量 : 0次 | 上传用户:snake_9655
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作者研究了过氧化氢气态等离子体灭活法灭活几种病毒的能力。用于实验的六种病毒包括:HIV病毒Ⅰ型、甲型肝炎病毒、呼吸道合胞病毒、牛痘病毒、单纯疱疹病毒Ⅰ型和脊髓灰质炎病毒Ⅱ型。把实验病毒悬浮于细胞培养液中,然后在无菌玻璃佩特里培养皿底部干燥。将接种过病毒的培养皿放入过氧化氢等离子体系统中进行灭活处理,处理时间是正常周期的一半。每种病毒接种四个培养皿,分别在两个单独的半周期进行实验,分析实验病毒的传染性和对指示细胞系的细胞毒性。在这些实验条件下,过氧化氢气态等离子体灭活过程产生对六种实验病毒的灭活,效果是病毒滴度下降幅度为10~(2.5)至10~(5.5),相当于99. The authors investigated the ability of hydrogen peroxide to inactivate several viruses by gaseous plasma inactivation. Six viruses used in the experiment include HIV virus type I, Hepatitis A virus, respiratory syncytial virus, vaccinia virus, herpes simplex virus type I, and poliovirus type II. The experimental virus was suspended in the cell culture medium and then dried on the bottom of a sterile glass Petri dish. The virus-inoculated dishes were placed in a hydrogen peroxide plasma system for inactivation for half the normal period. Four Petri dishes were inoculated with each virus and experiments were performed in two separate half-cycles to analyze the infectivity of the experimental virus and the cytotoxicity of the indicated cell lines. Under these experimental conditions, inactivation of six experimental viruses by gaseous hydrogen peroxide inactivation resulted in a drop in viral titer of 10 2.5 to 10 5.5, equivalent to 99.
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