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目的:观察慢性紫外线(UV)损伤对小鼠角质形成细胞(KC)角蛋白(CK)1和CK10表达的影响。方法:将实验小鼠随机分为空白对照组(20只)和UV损伤组(20只),采用UV光疗仪模拟日光中的长波紫外线(UVA)和中波紫外线(UVB)照射实验小鼠。照射从最小红斑量起始,UVB为0.07 J/cm~2,UVA为0.7 J/cm~2;每日1次,每周5 d,每周增加0.5个红斑量,连续照射9周,UVB累计照射剂量达9.45 J/cm~2,UVA累计照射剂量达94.5 J/cm~2。通过苏木精-伊红(HE)染色和间苯二酚-碱性品红染色(Weigert染色法)确定皮肤损伤,通过免疫组化方法分别对照射前(W0)、后(W9)CK1和CK10的表达进行检测。应用配对t检验和独立样本t检验对相关数据进行统计分析。结果:空白对照组小鼠W0及W9时,CK1和CK10的表达差异均无统计学意义(P均>0.05)。UV损伤组小鼠W0和W9时,CK1和CK10的表达差异均有统计学意义(P均<0.05)。且UV损伤组小鼠CK1和CK10的表达呈明显的定位改变。结论:多次UV照射能导致小鼠模型中CK1和CK10的表达明显上调且定位异常。
Objective: To observe the effect of chronic ultraviolet (UV) injury on the expression of keratin (CK) 1 and CK10 in keratinocytes of mice. Methods: The experimental mice were randomly divided into blank control group (20) and UV injury group (20). The experimental mice were irradiated by ultraviolet light (UVA) and ultraviolet light (UVB). UVB was 0.07 J / cm ~ 2 and UVA was 0.7 J / cm ~ 2 from the minimal erythema dose; once a day, once a week for 5 days, the number of erythema increased by 0.5 times a week for 9 weeks. UVB The accumulated radiation dose reached 9.45 J / cm ~ 2, and the cumulative radiation dose of UVA reached 94.5 J / cm ~ 2. Skin lesions were identified by hematoxylin and eosin (HE) staining and resorcinol-basic fuchsin staining (Weigert staining). Immunohistochemistry was used to detect the expression of CK1, CK10 expression was detected. The paired t-test and independent sample t-test were used for statistical analysis of the relevant data. Results: There was no significant difference in the expression of CK1 and CK10 between W0 and W9 in the blank control group (all P> 0.05). The expression of CK1 and CK10 in W0 and W9 mice in UV-damaged group were significantly different (all P <0.05). The expression of CK1 and CK10 in UV-damaged mice was obviously changed. Conclusion: Multiple UV irradiation can cause the expression of CK1 and CK10 in mouse model to be obviously up-regulated and the localization is abnormal.