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作者自行设计了一套小动物一氧化氮(NitricOxide,NO)吸入系统,该系统主要包括有机玻璃舱、采气泵、流量计、NO/N2混和气体、纯氧、氮氧化物分析仪、O2、CO2监测仪。实验过程中,将6-7只大鼠置于舱内分别吸入20ppm、40ppm、80ppm的NO8h,每20min记录一次舱内NO、NO2、O2和CO2的浓度值。结果表明舱内的实测NO浓度与实验所设计的NO浓度相近,舱内氧浓度与空气中的氧浓度相似,NO2和CO2最大浓度分别不超过3ppm和0.3%,而且在整个吸入过程中,舱内各气体浓度趋于稳定。该系统结构简单,使用方便,可用于大鼠长期吸入NO的实验研究作者自行设计了一套小动物一氧化氮(NitricOxide,NO)吸入系统,该系统主要包括有机玻璃舱、采气泵、流量计、NO/N2混和气体、纯氧、氮氧化物分析仪、O2、CO2监测仪。实验过程中,将6-7只大鼠置于舱内分别吸入20ppm、40ppm、80ppm的NO8h,每20min记录一次舱内NO、NO2、O2和CO2的浓度值。结果表明舱内的实测NO浓度与实验所设计的NO浓度相近,舱内氧浓度与空气中的氧浓度相似,NO2和CO2最大浓度分别不超过3ppm和?
The system designs a set of inhalation system of nitric oxide (NO) for small animals. The system mainly includes a plexiglass chamber, a gas collecting pump, a flow meter, a mixed gas of NO and N2, a pure oxygen and nitrogen oxide analyzer, CO2 monitor. During the experiment, 6-7 rats were inhaled into the chamber to inhale 20ppm, 40ppm, 80ppm of NO8h, record the concentration of NO, NO2, O2 and CO2 every 20min. The results show that the measured concentration of NO in the cabin is similar to that of the experimentally designed NO. The concentration of oxygen in the cabin is similar to the concentration of oxygen in the air. The maximum concentrations of NO2 and CO2 are no more than 3ppm and 0.3%, respectively. During the whole inhalation , The gas concentration in the cabin tends to be stable. The system is simple in structure and easy to use and can be used for long-term inhalation of NO in rats. The author designed a set of inhalation system of nitric oxide (NO) for small animals. The system mainly consists of a plexiglass chamber, a gas collecting pump, a flow meter , NO / N2 mixed gas, pure oxygen, nitrogen oxide analyzer, O2, CO2 monitor. During the experiment, 6-7 rats were inhaled into the chamber to inhale 20ppm, 40ppm, 80ppm of NO8h, record the concentration of NO, NO2, O2 and CO2 every 20min. The results show that the measured concentration of NO in the chamber is similar to that of the experimentally designed NO. The concentration of oxygen in the cabin is similar to that in air, and the maximum concentrations of NO2 and CO2 are not more than 3ppm and?