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M13噬菌体已被广泛地应用于噬菌体展示技术.M13的酶标抗体,是在噬菌体展示技术筛选结果ELISA检测中不可缺少的试剂.我们制备了兔抗M13抗血清,其效价高于1:6000;经两步饱和硫酸铵沉淀,初步纯化了兔抗M13抗体;用过碘酸钠法对抗体进行了辣根过氧化酶的标记,酶标记抗体活性为1:6400.
M13 phage has been widely used in phage display technology.M13 enzyme-labeled antibody is an indispensable reagent in the ELISA test of phage display technology screening results.We prepared rabbit anti-M13 antiserum with titer higher than 1: 6000 Rabbit anti-M13 antibody was purified by two-step saturated ammonium sulfate precipitation. Horseradish peroxidase (HRP) was labeled with sodium periodate, and the enzyme-labeled antibody activity was 1: 6400.