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目的 比较用异基因清髓性干细胞移植 (Allo SCT)和非清髓性干细胞移植 (NST)方法治疗狼疮鼠的有效性及安全性。方法 NST组 2 2只BXSB鼠用fludarabine +环磷酰胺 (CTX)作预处理。Allo SCT组 2 2只BXSB小鼠用马利兰 +CTX作预处理 ,预处理后受者输入经粒细胞集落刺激因子 (G CSF)动员后的供者 (C5 7BL/ 6 )的外周血干细胞 ,用环孢素A(CsA)、甲氨蝶呤 (MTX)预防移植物抗宿主病 (GVHD) ,G CSF促进移植后的造血和免疫重建 ,流式细胞仪检测供者Sca 1+ 含量及移植后受者T淋巴细胞亚群 (CD3-CD5 + 、CD3+ CD5 + )的变化 ,甲基纤维素半固体培养法培养供者的粒 巨噬细胞集落形成单位 (GM CFU) ,PCR法对移植后BXSB鼠嵌合状态进行分析 ,直接免疫荧光法和光镜检测移植前后受者的肾病理变化。结果 G CSF动员后不同时间两组供者外周血单个核细胞 (MNC)、CD+ 34、GM CFU数差异无显普性 (P均 >0 0 5 ) ,G CSF动员后第 7天两组供者MNC、CD34+ 、GM CFU数达最佳水平 ,NST组白细胞下降最低值分别为 (0 6± 0 2 )× 10 9/L ,高于Allo SCT组的 (0 2± 0 1)× 10 9/L (P <0 0 5 ) ,白细胞上升至 1 0× 10 9/L的时间在NST组为 (17 5±6 3)d ,比Allo SCT组明显缩短 (P <0 0 5 ) ,尿蛋白从 转为 +或 -、抗dsDNA抗体滴?
Objective To compare the efficacy and safety of allogeneic myeloablative stem cell transplantation (Allo SCT) and non-myeloablative stem cell transplantation (NST) in the treatment of lupus. Methods Twenty-two BXSB mice in the NST group were pretreated with fludarabine + cyclophosphamide (CTX). Allo SCT group 2 2 BXSB mice were pretreated with maryland + CTX. After pretreatment recipients received peripheral blood stem cells from donor (C5 7BL / 6) mobilized by granulocyte colony-stimulating factor (G CSF) Cyclosporine A (CsA), methotrexate (MTX) to prevent graft versus host disease (GVHD), G CSF to promote hematopoietic and immune reconstitution after transplantation, flow cytometry to detect donor Sca 1 + content and after transplantation (CD3-CD5 +, CD3 + CD5 +) of recipients were measured. GM CFU of donor was cultured by methylcellulose semi-solid culture method. The expression of Bcl-2 Rat chimerism status analysis, direct immunofluorescence and light microscopy before and after transplantation to detect renal pathological changes. Results There was no significant difference in the number of MNC, CD34, GM CFU between the two groups at different time points after G CSF mobilization (P> 0.05). On the 7th day after G CSF mobilization, (0 6 ± 0 2) × 10 9 / L, respectively, which was higher than that of Allo SCT group (0 2 ± 0 1) × 10 9 / L (P <0.05). The leukocyte up to 10 × 10 9 / L in the NST group was (17 5 ± 6 3) days, significantly shorter than that in the Allo SCT group (P 0 05) Protein from the switch to + or -, anti-dsDNA antibody drops?