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卵巢激素可调节脑、子宫、膀胱和肺内的β肾上腺素能受体;亦可作用于脑和膀胱的毒蕈碱样受体。因此推测,卵巢激素也可能作用于心脏的β肾上腺素能受体和毒蕈碱样受体。有人根据Baker和Potter介绍的方法制备心肌细胞膜,将膜的沉淀部分重新悬浮于60mol/L的磷酸钠钾缓冲液和50mmol/L的HEPES缓冲液中,分别用于毒蕈碱样受体和β肾上腺素能受体的结合测定。所得数据全部用微机处理,并通过直线回归分析和Scathacrd作图得到结合常数、解离常数和受体密度等分析参数。离体实验表明,所有的孕激素,只要浓度达到100μmol/L都可明显抑制~3H-二苯羟乙酸奎宁酯(~3H-QNB)与去卵巢大鼠心肌毒蕈碱样受体的结合,其中以孕酮和16α-甲基孕酮的作用最强,并呈现快速、可逆性的剂量依赖式抑制;而雌激
Ovarian hormones regulate the beta adrenergic receptors in the brain, uterus, bladder and lungs; they also act on muscarinic receptors in the brain and bladder. Therefore, we hypothesize that ovarian hormones may also act on beta adrenergic receptors and muscarinic receptors in the heart. Cardiomyocytes membranes were prepared according to the method described by Baker and Potter. The pellet was partially resuspended in 60 mol / L potassium phosphate buffer and 50 mmol / L HEPES buffer for muscarinic receptors and β Adrenergic receptor binding assay. The data obtained were all processed by computer, and the analytical parameters such as binding constant, dissociation constant and acceptor density were obtained by linear regression analysis and Scathacrd mapping. In vitro experiments showed that all the progestins, as long as the concentration of 100μmol / L can significantly inhibit the ~ 3H-benzidine (~ 3H-QNB) and ovariectomy myocardial muscarinic receptor binding , Of which progesterone and 16α-methyl progestin strongest role, and showed rapid, reversible dose-dependent inhibition; and estrogen