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目的研究青枯菌诱导广藿香的致病过程及防御相关酶同工酶的动态变化。方法利用青枯菌粗毒素诱导广藿香试管苗,并用聚丙烯酰胺凝胶电泳法,对诱导植株中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)同工酶谱带的变化进行分析。结果青枯菌诱导1～7 d后的广藿香植株,表现渐进的发病过程,开始时,植株失绿、少数叶片萎垂;逐渐植株茎杆弯曲、整株叶片萎蔫。同工酶电泳分析表明,SOD同工酶在第1、3天时分别出现了新谱带,与对照共有的谱带,强度先增后减;CAT同工酶在第3、5天时分别出现新谱带,第6天时强度达到最大;POD同工酶在第1、4天时分别出现了新谱带,强度先增后减,第7天时所有谱带消失。结论青枯病的发生呈现渐进的过程。青枯菌诱导1～7 d,广藿香SOD、CAT和POD同工酶谱带在数目和强度上均有所不同,呈动态变化,表明SOD、CAT和POD在广藿香抵抗青枯菌入侵时可能起到较为重要的作用。
Objective To study the pathogenicity of Pogostemon cablin induced by R. solanacearum and the dynamic changes of defense enzymes. Methods The in vitro culture of Pogostemon cablin was induced by Ralstonia solanacearum crude toxin and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) Isoenzyme bands were analyzed for changes. Results Ralstonia solanacearum was induced patchouli plants from 1 to 7 days, showing progressive pathogenesis. At the beginning, the plants lost their green color and a few of them wilted. Gradual plant stems were bent and the whole plant wilted. Isoenzyme electrophoresis analysis showed that SOD isozymes appeared new bands on the 1st and 3rd day, respectively, and the bands shared with the control increased firstly and then decreased; CAT isozymes appeared new on the 3rd and 5th day, respectively The bands reached the maximum on the 6th day. The POD isozymes showed new bands on the 1st and the 4th days respectively, the intensity increased first and then decreased, and disappeared on the 7th day. Conclusion The occurrence of bacterial wilt showed a gradual process. The bands of SOD, CAT and POD in Pogostemon cablin were different in the number and intensity of 1 ~ 7 d after induced by R. solanacearum, showing dynamic changes of SOD, CAT and POD, Invasion may play a more important role.