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硒对自发性乳腺癌有抑制作用,可抑制移植性肝癌(腹水型)的生长。硒摄取量与多种肿瘤发病率呈明显的负相关。有人还发现,癌症患者血硒含量低于正常人,血硒越低,预后也越差。这些结果表明,硒有控制癌症的作用,但其作用机理尚不明了。对肿瘤生长增殖有重要调控作用。本文观察亚硒酸钠对肝癌、正常肝和宿主肝CAMP含量的影响,以探讨硒的作用机理。材料和方法(一)试剂~3H-cAMP(27Ci/mmol),~3H-脱氧胸苷(~3H-TdR,37Ci/mmol)上海原子核研究所产品。cAMP,二丁酰基-cAMP(DB-cAMP,Boehringer),Macoy 5′A培养液由本所细胞生物室供给。蛋白激酶由牛肌肉中提取。微孔滤膜(φ=2.5cm),孔径为 0.3～0.45μm。其余试剂为北京化学
Selenium has an inhibitory effect on spontaneous breast cancer and inhibits the growth of transplanted liver cancer (ascites type). Selenium intake was significantly negatively correlated with the incidence of multiple tumors. Some people also found that the blood selenium content of cancer patients is lower than normal, the lower the blood selenium, the worse the prognosis. These results show that selenium has a role in controlling cancer, but its mechanism of action is still unknown. It plays an important role in regulating tumor growth and proliferation. In this paper, the effect of sodium selenite on the CAMP content of liver cancer, normal liver and host liver was observed to explore the mechanism of selenium. Materials and Methods (1) Reagents ~3H-cAMP (27Ci/mmol), ~3H-deoxythymidine (~3H-TdR, 37Ci/mmol) Shanghai Institute of Nuclear Research Products. The cAMP, dibutyryl-cAMP (DB-cAMP, Boehringer), Macoy 5’A broth was supplied by our cell biology room. Protein kinases are extracted from bovine muscle. Microporous membrane (φ=2.5cm), pore size 0.3 to 0.45μm. The remaining reagents are Beijing Chemicals