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以甘春24、甘春20和中麦175为材料,通过植物组织培养技术,研究了不同浓度CuSO_4和EDTAFe对小麦成熟胚再生体系的影响。结果表明,在诱导及分化培养基中适当增加CuSO_4浓度(0.5~10μmol/L),显著提高小麦成熟胚愈伤组织的出愈率、胚性愈伤率、分化率和绿苗率。在CuSO_4最适浓度(10μmol/L)下,甘春24、甘春20和中麦175的出愈率、胚性愈伤率、分化率和绿苗率分别比对照(CuSO_4浓度为0.5μmol/L)提高了62.82%、78.68%和31.04%,64.57%、66.01%和49.92%,77.40%、77.93%和52.45%,86.60%、69.85%和67.39%。培养基中添加70μmol/L EDTA-Fe均有利于三个小麦品种成熟胚愈伤组织的形成和分化,尤其对中麦175的培养效果最好。在EDTA-Fe最适浓度(70μmol/L)下,甘春24、甘春20和中麦175的出愈率、胚性愈伤率、分化率和绿苗率分别比对照(EDTA-Fe浓度为50μmol/L)提高了38.66%、32.27%和21.05%,42.10%、36.92%和25.00%,42.63%、57.42%和32.25%,40.02%、53.11%和45.11%。当EDTA-Fe浓度高于70μmol/L时,三个小麦品种出愈率等均呈现下降趋势,说明其浓度过高会对小麦愈伤组织造成伤害。不同小麦基因型对相同浓度CuSO_4和EDTA-Fe敏感性不一致,在相同培养条件下,中麦175成熟胚的愈伤组织形成和分化情况最好,甘春24次之,甘春20最低。
Taking Ganchun 24, Ganchun 20 and Zhongmai 175 as materials, the effects of different concentrations of CuSO4 and EDTAFe on the regeneration system of wheat mature embryos were studied by plant tissue culture technology. The results showed that the CuSO 4 concentration (0.5 ~ 10μmol / L) was increased in the induction and differentiation medium, and the callus formation, embryogenic callus, differentiation rate and green shoot rate of wheat embryos were significantly increased. Cucurbitacin 24, Ganchun 20 and Zhongmai 175 were higher than that of the control (CuSO 4 concentration 0.5μmol / L) at CuSO 4 optimum concentration (10μmol / L) L increased by 62.82%, 78.68% and 31.04%, 64.57%, 66.01% and 49.92%, 77.40%, 77.93% and 52.45%, 86.60%, 69.85% and 67.39%, respectively. The addition of 70μmol / L EDTA-Fe to the medium was beneficial to the formation and differentiation of the mature embryo callus of three wheat cultivars, especially for Zhongmai 175. In the optimum concentration of EDTA-Fe (70μmol / L), the callus, embryogenic callus, differentiation rate and green shoot rate of Ganchun 24, Ganchun 20 and Zhongmai 175 were higher than those of the control (EDTA-Fe concentration Was 38.66%, 32.27% and 21.05%, 42.10%, 36.92% and 25.00%, 42.63%, 57.42% and 32.25%, 40.02%, 53.11% and 45.11% higher than that of the control (50μmol / L) When EDTA-Fe concentration was higher than 70μmol / L, the emergence rate of three wheat varieties showed a downward trend, indicating that the high concentration of wheat callus will cause injury. Different wheat genotypes were not consistent with the same concentration of CuSO 4 and EDTA-Fe. Under the same culture conditions, the callus formation and differentiation of Zhongmai 175 mature embryo was the best, with Ganchun 24 times and Ganchun 20 the lowest.